Berridge M V, Ralph R K, Letham D S
Biochem J. 1970 Aug;119(1):75-84. doi: 10.1042/bj1190075.
The synthetic cytokinins kinetin and 6-benzylaminopurine exhibit equilibrium-type binding to purified chinese-cabbage leaf ribosomes. At 23mum and 4 degrees C one molecule of kinetin and 1.34 molecules of 6-benzylaminopurine are bound per ribosome. Adenine and adenine derivatives that are inactive as cytokinins showed much less affinity for ribosomes. Pretreatment of ribosomes with 0.5m-ammonium chloride or Triton X-100 did not decrease the extent of cytokinin binding. Binding appeared to be to the 83S ribosome species. A positive correlation between the extent of binding and the biological effect of various cytokinin analogues was demonstrated. These results are discussed in terms of cytokinin control of growth processes at the ribosomal level.
合成细胞分裂素激动素和6-苄基腺嘌呤与纯化的大白菜叶片核糖体呈现平衡型结合。在23μm和4℃时，每个核糖体结合一分子激动素和1.34分子6-苄基腺嘌呤。作为细胞分裂素无活性的腺嘌呤和腺嘌呤衍生物对核糖体的亲和力要低得多。用0.5m氯化铵或曲拉通X-100预处理核糖体不会降低细胞分裂素的结合程度。结合似乎发生在83S核糖体种类上。各种细胞分裂素类似物的结合程度与生物学效应之间呈正相关。从核糖体水平上细胞分裂素对生长过程的控制方面对这些结果进行了讨论。
