Voigt R, Korner I, Roth P
Pharmazie. 1979 Jan;34(1):45-8.
Equilibrium dialyses and fluorescent spectroscopic measurements demonstrated that human serum albumin (HSA) affects the oxytetracycline (OTC)--polyvinyl-pyrrolidone K 90 (PVP) interactions. The preferential binding of OTC with HSA, as compared to PVP, was evidenced by means of a three-compartment model. The binding values increased with the increase of the portion of PVP in the PVP-HSA mixture. Superadditive OTC bindings occurred at a ratio of 1:1 (by weight). The addition of Mg2+ to PVP-containing solutions, at a molar ratio of 1:1, also produced an increase of the interactions of this antibiotic with PVP, whereby maximal OTC-PVP bindings of 50--60% were reached.
平衡透析和荧光光谱测量表明，人血清白蛋白（HSA）会影响土霉素（OTC）与聚乙烯吡咯烷酮K90（PVP）之间的相互作用。通过三室模型证明，与PVP相比，OTC与HSA具有优先结合。结合值随着PVP-HSA混合物中PVP比例的增加而增加。当比例为1:1（按重量）时，OTC发生超加成结合。以1:1的摩尔比向含PVP的溶液中添加Mg2+，也会使这种抗生素与PVP之间的相互作用增加，从而使OTC与PVP的最大结合率达到50%-60%。
