Yi-hsun L
Sci Sin. 1979 Feb;22(2):222-35.
This paper presents evidence to show the presence of an oestrogen-induced protein (IP) from rat uterus which is able to stimulate the incorporation of 3H-thymidine into DNA of 3T6 fibroblasts in vitro. The purified uterine protein fraction has been shown th contain less than 10 detectable proteins. The molecular weight of IP band is about 50,000 Daltons with an iso-electric point of 4.5. Comparison of oestrogen-treated and -untreated uterine cytosols on SDS-acrylamide gel electrophoresis indicates that the IP band is also present in the untreated controls. It is therefore suggested that the oestrogen-induced protein may be analogous or identical to the growth-promoting protein synthesized by LX cells.
本文提供证据表明，大鼠子宫中存在一种雌激素诱导蛋白（IP），该蛋白能够在体外刺激3H-胸腺嘧啶核苷掺入3T6成纤维细胞的DNA中。已证明纯化的子宫蛋白组分含有少于10种可检测到的蛋白质。IP条带的分子量约为50,000道尔顿，等电点为4.5。SDS-聚丙烯酰胺凝胶电泳对雌激素处理和未处理的子宫胞质溶胶的比较表明，未处理的对照中也存在IP条带。因此，有人提出雌激素诱导蛋白可能与LX细胞合成的促生长蛋白类似或相同。
