Tsai M J, Tsai S Y, O'Malley B W
Science. 1979 Apr 20;204(4390):314-6. doi: 10.1126/science.432646.
A study was made of the function of the intervening sequences in the ovalbumin gene, Radioactively labeled DNA probes for the intervening sequences were prepared and RNA's were isolated from whole cells, nuclei, and polysomes of estrogen-stimulated chick oviducts. The concentrations of messenger RNA (mRNA) transcripts from ovalbumin structural sequences (mRNAov) and transcripts corresponding to intervening sequences were then estimated by hybridization to cloned DNA probes. Oviduct tissue contains approximately 58,000 molecules of mRNAov sequences per tubular gland cell and most of these sequences are present in the cytoplasm. In contrast, there are 200 to 300 molecules of RNA per cell which are transcribed from the intervening sequences of the natural ovalbumin gene and almost all of these are found in the nucleus. The difference in distribution of structural and intervening sequence transcripts suggests that, unlike mature mRNA, the intervening sequences are not preferentially transported to cytoplasmic polysomes.
对卵清蛋白基因中居间序列的功能进行了一项研究。制备了用于居间序列的放射性标记DNA探针，并从雌激素刺激的鸡输卵管的全细胞、细胞核和多核糖体中分离出RNA。然后通过与克隆的DNA探针杂交来估计来自卵清蛋白结构序列的信使RNA（mRNA）转录本（mRNAov）的浓度以及与居间序列相对应的转录本的浓度。输卵管组织每个管状腺细胞中约含有58,000个mRNAov序列分子，并且这些序列中的大多数存在于细胞质中。相比之下，每个细胞中有200到300个从天然卵清蛋白基因的居间序列转录而来的RNA分子，并且几乎所有这些分子都存在于细胞核中。结构序列和居间序列转录本分布的差异表明，与成熟mRNA不同，居间序列不会优先转运到细胞质多核糖体中。
