Dugaiczyk A, Woo S L, Lai E C, Mace M L, McReynolds L, O'Malley B W
Nature. 1978 Jul 27;274(5669):328-33. doi: 10.1038/274328a0.
EcoRI fragments of the natural ovalbumin gene were cloned and studied by hybridisation with mature ovalbumin mRNA, electron microscopy, restriction enzyme mapping and limited sequence analysis. The structural gene sequences coding for ovalbumin were found to be separated into eight sequentially orientated pieces by seven intervening sequences of various lengths.
天然卵清蛋白基因的EcoRI片段通过与成熟卵清蛋白mRNA杂交、电子显微镜观察、限制性酶切图谱分析和有限序列分析进行克隆和研究。发现编码卵清蛋白的结构基因序列被七个不同长度的间隔序列分隔成八个顺序排列的片段。