Wyss C
Somatic Cell Genet. 1979 Jan;5(1):23-8. doi: 10.1007/BF01538783.
Yeast extract, a component of Drosophila cell culture media, is shown to contain substances of high, intermediate, and low molecular weight, that are, respectively, essential, inhibitory, and stimulatory for colony formation in semisolid agar medium. Furthermore, it is shown that high concentrations of pyridoxal greatly increase the cloning efficiency of Drosophilia cells. A cloning method with line Kc is described which routinely gives cloning efficiencies in excess of 20%.
酵母提取物是果蝇细胞培养基的一种成分，已表明它含有高分子量、中等分子量和低分子量的物质，这些物质分别对半固体琼脂培养基中的集落形成是必需的、有抑制作用的和有刺激作用的。此外，还表明高浓度的吡哆醛能大大提高果蝇细胞的克隆效率。本文描述了一种用Kc系的克隆方法，该方法通常能产生超过20%的克隆效率。
