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1
Preparative electrophoresis of isotopically labeled L-cell interferons.同位素标记的L细胞干扰素的制备电泳
Appl Microbiol. 1971 Jun;21(6):1067-71. doi: 10.1128/am.21.6.1067-1071.1971.
2
Characterization of interferon-associated proteins.干扰素相关蛋白的特性分析
J Gen Virol. 1972 May;15(2):129-38. doi: 10.1099/0022-1317-15-2-129.
3
Blocking of interferon production by chromatographically purified L cell interferon.经色谱纯化的L细胞干扰素对干扰素产生的阻断作用。
Proc Soc Exp Biol Med. 1973 Jan;142(1):167-74. doi: 10.3181/00379727-142-36981.
4
Purification, characterization, and attempts at isotopic labeling of mouse interferon.小鼠干扰素的纯化、特性鉴定及同位素标记尝试
J Virol. 1970 Feb;5(2):145-52. doi: 10.1128/JVI.5.2.145-152.1970.
5
Activity of mouse interferon in human cells.小鼠干扰素在人细胞中的活性。
Proc Soc Exp Biol Med. 1971 Sep;137(4):1392-5. doi: 10.3181/00379727-137-35796.
6
Partial purification and characterization of Syrian hamster interferon.叙利亚仓鼠干扰素的部分纯化及特性分析
J Gen Virol. 1981 Feb;52(Pt 2):227-33. doi: 10.1099/0022-1317-52-2-227.
7
Intracellular interferon: kinetics of formation and release.细胞内干扰素：形成与释放的动力学
Virology. 1971 Sep;45(3):837-40. doi: 10.1016/0042-6822(71)90209-1.
8
Purification of mouse and human interferons: detection of subunit structure.小鼠和人干扰素的纯化：亚基结构的检测
Prep Biochem. 1971 Jan;1(1):55-75. doi: 10.1080/00327487108081930.
9
Distinguishing characteristics of the interferon responses of primary and continuous mouse cell cultures.原代和连续传代小鼠细胞培养物干扰素反应的鉴别特征。
J Gen Virol. 1971 Oct;13(1):35-50. doi: 10.1099/0022-1317-13-1-35.
10
Purification of mouse L cell interferon. Essentially pure preparations with associated cell growth inhibitory activity.小鼠L细胞干扰素的纯化。具有相关细胞生长抑制活性的基本纯品制剂。
J Biol Chem. 1978 Jul 25;253(14):5074-9.

本文引用的文献

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DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.圆盘电泳。II. 方法及其在人血清蛋白中的应用。
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Polyacrylamide gel electrophoresis of highly purified chick interferon.高纯度鸡干扰素的聚丙烯酰胺凝胶电泳
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Isoelectric fractionation, analysis, and characterization of ampholytes in natural pH gradients. IV. Further studies on the resolving power in connection with separation of myoglobins.天然pH梯度中两性电解质的等电分级分离、分析及特性鉴定。IV. 与肌红蛋白分离相关的分辨率的进一步研究。
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Properties of rat-tumor interferon.大鼠肿瘤干扰素的特性。
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5
Soybean inhibitors. II. Preparative electrophoretic purification of soybean proteinase inhibitors on polyacrylamide gel.大豆抑制剂。II. 聚丙烯酰胺凝胶上大豆蛋白酶抑制剂的制备性电泳纯化
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Gel electrophoresis: a new catalyst for acid systems.凝胶电泳：酸性体系的新型催化剂。
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7
Fractionation of oligodeoxynucleotides by polyacrylamide gel electrophoresis.通过聚丙烯酰胺凝胶电泳对寡脱氧核苷酸进行分级分离。
Anal Biochem. 1969 Feb;27(2):193-204. doi: 10.1016/0003-2697(69)90023-2.
8
Chick interferon: heterogeneity of electric charge.
Science. 1969 Mar 14;163(3872):1198-9. doi: 10.1126/science.163.3872.1198.
9
Analytical and preparative separation of acidic glycosaminoglycans by electrophoresis in barium acetate.在醋酸钡中通过电泳对酸性糖胺聚糖进行分析性和制备性分离。
Anal Biochem. 1968 Dec;26(3):439-44. doi: 10.1016/0003-2697(68)90205-4.
10
Isoelectric components of mouse, human, and rabbit interferons.小鼠、人及兔干扰素的等电成分。
Virology. 1970 Aug;41(4):740-50. doi: 10.1016/0042-6822(70)90438-1.

同位素标记的L细胞干扰素的制备电泳

Preparative electrophoresis of isotopically labeled L-cell interferons.

作者信息

Stancek D, Paucker K

出版信息

Appl Microbiol. 1971 Jun;21(6):1067-71. doi: 10.1128/am.21.6.1067-1071.1971.

DOI:10.1128/am.21.6.1067-1071.1971
PMID:4327610
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC377345/
Abstract

The preparative method of polyacrylamide gel electrophoresis was adapted for purification and characterization of isotopically labeled L-cell interferons. Re-covery of interferon activity was quantitative, and purification and resolution were comparable to those obtained by analytical polyacrylamide gel electrophoresis. Ultimate specific activities attainable ranged from 2 x 10(6) to 3 x 10(6) international units per mg of protein.

摘要

聚丙烯酰胺凝胶电泳的制备方法被用于纯化和鉴定同位素标记的L细胞干扰素。干扰素活性的回收率是定量的，其纯化和分辨率与分析性聚丙烯酰胺凝胶电泳所获得的相当。每毫克蛋白质可达到的最终比活性范围为2×10⁶至3×10⁶国际单位。