Kim I C, Bragg P D
J Bacteriol. 1971 Sep;107(3):664-70. doi: 10.1128/jb.107.3.664-670.1971.
The reaction with o-phenanthroline of nonheme iron found in cell envelope fractions from Escherichia coli has been investigated. About 20% of the total nonheme iron reacts directly with o-phenanthroline. This iron appears to be in the ferric state but is reducible by protein sulfhydryl groups in the presence of the chelating agent. A further 20% of the nonheme iron will react with o-phenanthroline only in the presence of dithionite. Succinate can replace dithionite but only produces about 35% of the reaction given by dithionite. The reduction of cytochrome b(1) of the respiratory chain by succinate shows similar behavior to the reaction of iron with o-phenanthroline in the presence of succinate. Both of these components react completely only in the presence of 2-heptyl-4-hydroxyquinoline-N-oxide. The remaining 60% of the nonheme iron of the cell envelope will not react with o-phenanthroline even in the presence of dithionite or 6 m urea. Triton X-100 with dithionite will permit a small part (10%) of this iron to react with o-phenanthroline. The iron which does not react with o-phenanthroline is not associated with succinate, reduced nicotinamide adenine dinucleotide, or d-lactate dehydrogenases.
对大肠杆菌细胞膜组分中发现的非血红素铁与邻菲罗啉的反应进行了研究。约20%的总非血红素铁直接与邻菲罗啉反应。这种铁似乎处于三价铁状态，但在螯合剂存在下可被蛋白质巯基还原。另外20%的非血红素铁仅在连二亚硫酸盐存在时才会与邻菲罗啉反应。琥珀酸盐可替代连二亚硫酸盐，但产生的反应仅为连二亚硫酸盐的约35%。琥珀酸盐对呼吸链细胞色素b(1)的还原显示出与琥珀酸盐存在时铁与邻菲罗啉反应类似的行为。这两种成分仅在2-庚基-4-羟基喹啉-N-氧化物存在时才会完全反应。细胞膜剩余60%的非血红素铁即使在连二亚硫酸盐或6M尿素存在下也不会与邻菲罗啉反应。连二亚硫酸盐存在时的Triton X-100会使这部分铁中的一小部分（10%）与邻菲罗啉反应。不与邻菲罗啉反应的铁与琥珀酸盐、还原型烟酰胺腺嘌呤二核苷酸或d-乳酸脱氢酶无关。
