Moore G L, Ledford M E, Brooks D E
Transfusion. 1979 Jan-Feb;19(1):68-71. doi: 10.1046/j.1537-2995.1979.19179160270.x.
Eight units of blood were drawn into modified CPD containing 25 per cent higher glucose and 17.3 mg adenine (0.25 mM in blood). Red blood cell concentrates (RCC) were prepared to a mean hematocrit (Hct) of 70, the cells stored at 4 C, and plasma adenine and red blood cell adenosine triphosphate (ATP) were measured weekly for 42 days. The removal of plasma in the preparation of RCC reduced by 39 per cent the available adenine. As a result measurable plasma adenine was depleted by 21 days. The loss of ATP in RCC occurs at a significantly faster rate than in whole blood stored under the same conditions. When red blood cells are stored at higher HCT or for periods longer than 35 days, increased anticoagulant adenine levels are recommended.
将8单位血液采集到含有比正常浓度高25%葡萄糖和17.3毫克腺嘌呤（血液中为0.25毫摩尔）的改良枸橼酸盐-磷酸盐-葡萄糖（CPD）抗凝剂中。制备平均血细胞比容（Hct）为70%的红细胞浓缩液（RCC），细胞保存在4℃，每周测定血浆腺嘌呤和红细胞三磷酸腺苷（ATP）含量，持续42天。制备RCC时去除血浆使可利用的腺嘌呤减少了39%。结果，可检测到的血浆腺嘌呤在21天时耗尽。RCC中ATP的损失速度比在相同条件下储存的全血快得多。当红细胞以更高的血细胞比容储存或储存时间超过35天时，建议提高抗凝剂中腺嘌呤的水平。
