Webert D W, Cohen D
Appl Microbiol. 1971 Jul;22(1):66-78. doi: 10.1128/am.22.1.66-78.1971.
An automated hemagglutination-inhibition (HI) test for the "shipping fever" strain (SF-4) of parainfluenza 3 antibody in bovine sera was developed and compared to manual tube and microtiter test procedures. The automated system operating at 60 samples per hr provided the most test results per specified time period, and the manual tube test provided the least. The manual microtiter test and the automated system at 40 samples per hr, falling between the two above procedures, were comparable in the number of sera that could be titrated in 1 day by one technician. There was little difference between automated and manual test reproducibility when measured at the twofold titer one-dilution difference level. However, the automated system titrated a higher number of sera at the same titer on repeat runs than either of the manual test procedures. The automated one-quartile difference reproducibility (each twofold dilution subdivided into 4 units-"quartiles") was equal to the manual test one-dilution difference reproducibility. The standard deviation of the per cent variation from the mean of paired serum titers for 40-sample-per-hr runs ranged from +/-3.49 to +/-5.36%. The manual and automated systems were of comparable sensitivity in their detection of negative sera.
开发了一种用于检测牛血清中副流感3抗体“运输热”毒株（SF-4）的自动血凝抑制（HI）试验，并将其与手动试管法和微量滴定法进行了比较。每小时处理60个样本的自动系统在规定时间内提供的检测结果最多，而手动试管法提供的最少。每小时处理40个样本的手动微量滴定法和自动系统介于上述两种方法之间，一名技术人员在一天内可滴定的血清数量相当。在两倍滴度的一个稀释度差异水平下测量时，自动检测和手动检测的重复性差异不大。然而，在重复运行中，自动系统在相同滴度下滴定的血清数量比任何一种手动检测方法都多。自动四分位数差异重复性（每个两倍稀释度细分为4个单位——“四分位数”）与手动检测的一个稀释度差异重复性相当。每小时处理40个样本的运行中，配对血清滴度均值的变异百分比标准差在±3.49%至±5.36%之间。手动和自动系统在检测阴性血清方面的灵敏度相当。
