用于检测牛血清中针对副流感3型病毒、牛传染性鼻气管炎病毒和牛病毒性腹泻病毒抗体的微量滴定试验。
Microtiter tests for detecting antibody in bovine serum to parainfluenza 3 virus, infectious bovine rhinotracheitis virus, and bovine virus diarrhea virus.
作者信息
Rossi C R, Kiesel G K
出版信息
Appl Microbiol. 1971 Jul;22(1):32-6. doi: 10.1128/am.22.1.32-36.1971.
Microneutralization tests for detection of antibody in bovine serum to three bovine viruses are described. The Madin-Darby bovine kidney cell line was used with parainfluenza 3 virus (PI 3), whereas serially cultivated bovine embryonic kidney cells were used for infectious bovine rhinotracheitis virus and bovine virus diarrhea virus. Comparison of micro-hemagglutination-inhibition (HI) with micro-serum-neutralization (SN) tests for PI 3 showed the SN test to be more sensitive, more specific, and therefore more useful than the HI test for detecting antibody. Although the effect of trypsin-periodate treatment of serum was to reduce the HI titer of numerous sera by a twofold dilution, sufficient evidence could not be found to indicate that nonspecific HI inhibitors to PI 3 are present in bovine sera.
本文描述了用于检测牛血清中针对三种牛病毒抗体的微量中和试验。使用马迪-达比牛肾细胞系检测副流感3病毒（PI 3），而连续培养的牛胚胎肾细胞用于检测传染性牛鼻气管炎病毒和牛病毒性腹泻病毒。对PI 3进行微量血凝抑制（HI）试验和微量血清中和（SN）试验的比较表明，SN试验在检测抗体方面比HI试验更敏感、更特异，因此更有用。尽管用胰蛋白酶-高碘酸盐处理血清会使许多血清的HI效价降低两倍稀释度，但未发现足够证据表明牛血清中存在针对PI 3的非特异性HI抑制剂。
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