粗糙脉孢菌中的一种类晶体包涵体。由溴化乙锭和吖啶诱导产生、分离及特性研究。
A paracrystalline inclusion in Neurospora crassa. Induction by ethidium and acridine, isolation, and characterization.
作者信息
Wood D D, Luck D J
出版信息
J Cell Biol. 1971 Oct;51(1):249-64. doi: 10.1083/jcb.51.1.249.
A paracrystal indistinguishable from the one which occurs in the mitochondrial mutant abnormal-1 can be induced in wild-type Neurospora crassa after growth in either ethidium or euflavine. This paracrystal has been isolated and partially characterized. It appears to be composed of a single polypeptide (mol wt 68,000) which can be reversibly crystallized and dissociated by changes in the pH and ionic strength. When aggregated, the polypeptide forms oligomers which are arranged end-to-end into fibers. During the characterization of the polypeptide, it was found that the polypeptide's electrophoretic and immunological properties could be used as assays. Using these methods it was found that the polypeptide normally accumulates in a soluble form in the cytoplasm of wild-type Neurospora at the end of the log-phase of growth.
在野生型粗糙脉孢菌中，当在溴化乙锭或优黄素中生长后，可诱导出一种与线粒体突变体abnormal-1中出现的副晶体无法区分的副晶体。这种副晶体已被分离并进行了部分表征。它似乎由单一多肽（分子量68,000）组成，该多肽可通过pH值和离子强度的变化可逆地结晶和解离。聚集时，多肽形成寡聚体，这些寡聚体首尾相连排列成纤维。在对该多肽进行表征的过程中，发现该多肽的电泳和免疫特性可用作分析方法。使用这些方法发现，在对数生长期结束时，该多肽通常以可溶形式积累在野生型粗糙脉孢菌的细胞质中。
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