脑组织中一种独特的突触体组分，它能积累谷氨酸和天冬氨酸。
A unique synaptosomal fraction, which accumulates glutamic and aspartic acids, in brain tissue.
作者信息
Wofsey A R, Kuhar M J, Snyder S H
出版信息
Proc Natl Acad Sci U S A. 1971 Jun;68(6):1102-6. doi: 10.1073/pnas.68.6.1102.
Abstract
Subcellular fractionation of rat cerebral cortical slices on sucrose density gradients provides evidence for the existence of a unique synaptosomal fraction (enriched in pinched-off nerve endings) that selectively accumulates glutamic and aspartic acids. The particles in this fraction sediment to a less dense portion of sucrose gradients than do particles that accumulate aromatic, basic, and neutral (large and small) amino acids. Particles that store gamma-aminobutyric acid are even less dense than those that contain exogenous glutamic and aspartic acids. The distribution of endogenous glutamic acid encompasses both that of exogenous glutamic acid and that of the neutral and basic amino acids. These findings provide neurochemical support for the suggestion that glutamic and/or aspartic acid has a specialized synaptic function, perhaps as a neurotransmitter, in the mammalian brain.
摘要
在蔗糖密度梯度上对大鼠大脑皮质切片进行亚细胞分级分离，为一种独特的突触体组分（富含 pinched-off 神经末梢）的存在提供了证据，该组分选择性地积累谷氨酸和天冬氨酸。与积累芳香族、碱性和中性（大、小）氨基酸的颗粒相比，该组分中的颗粒沉淀到蔗糖梯度密度较低的部分。储存γ-氨基丁酸的颗粒甚至比含有外源性谷氨酸和天冬氨酸的颗粒密度更低。内源性谷氨酸的分布既包括外源性谷氨酸的分布，也包括中性和碱性氨基酸的分布。这些发现为谷氨酸和/或天冬氨酸在哺乳动物大脑中具有特殊的突触功能（可能作为神经递质）这一观点提供了神经化学支持。
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