葡萄球菌核酸酶的折叠：单个残基的磁共振和荧光研究
Folding of staphylococcal nuclease: magnetic resonance and fluorescence studies of individual residues.
作者信息
Epstein H F, Schechter A N, Cohen J S
出版信息
Proc Natl Acad Sci U S A. 1971 Sep;68(9):2042-6. doi: 10.1073/pnas.68.9.2042.
Abstract
The reversible unfolding and folding of staphylococcal nuclease in the acid transition has been studied by 220 MHz proton magnetic resonance spectroscopy. The values of area, line-width, and chemical shift of each of the imidazole C2 proton resonances of the four histidine residues have been measured in this transition. The change of areas of three histidine resonances and the change of fluorescence of the single tryptophan residue, as a function of pH, appear to follow a single equilibrium. In contrast, a fourth histidine resonance follows a biphasic transition. These findings indicate that local conformational changes can be detected by magnetic resonance spectroscopy in the cooperative transition of the overall structure.
摘要
通过220兆赫质子磁共振波谱研究了葡萄球菌核酸酶在酸性转变过程中的可逆去折叠和折叠。在该转变过程中测量了四个组氨酸残基中每个咪唑C2质子共振的面积、线宽和化学位移值。三个组氨酸共振面积的变化以及单个色氨酸残基荧光的变化作为pH的函数，似乎遵循单一平衡。相比之下，第四个组氨酸共振遵循双相转变。这些发现表明，在整体结构的协同转变中，可以通过磁共振波谱检测到局部构象变化。
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3
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4
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6
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