Levinson B B, Baxter J D, Rousseau G G, Tomkins G M
Science. 1972 Jan 14;175(4018):189-90. doi: 10.1126/science.175.4018.189.
The cellular site of binding of dexamethasone by specific glucocorticoid receptors in cultured hepatoma cells was investigated with the use of certain mercurials. p-Chloromercuribenzene sulfonate and p-chloromercuribenzoate inhibit the binding of steroid by receptors in cell-free extracts, but they allow the steroid-receptor complex to form in whole cells. In contrast, HgCl(2) inhibits binding both in extracts and cells. Since both organic mercury compounds, unlike HgCl(2), do not readily enter intact cells, it appears that the specific steroid binding occurs inside the cell rather than at the cell membrane.
利用某些汞制剂研究了培养的肝癌细胞中地塞米松与特异性糖皮质激素受体的细胞结合位点。对氯汞苯磺酸盐和对氯汞苯甲酸可抑制无细胞提取物中受体与类固醇的结合，但它们能使类固醇-受体复合物在完整细胞中形成。相比之下，HgCl₂在提取物和细胞中均抑制结合。由于与HgCl₂不同，这两种有机汞化合物不易进入完整细胞，因此看来特异性类固醇结合发生在细胞内而非细胞膜上。
