一种来自哺乳动物细胞的能解开超螺旋DNA的活性物质——一种可能用于DNA复制的旋转酶（多瘤病毒-溴化乙锭-小鼠胚胎细胞-染料结合测定）
An activity from mammalian cells that untwists superhelical DNA--a possible swivel for DNA replication (polyoma-ethidium bromide-mouse-embryo cells-dye binding assay).
作者信息
Champoux J J, Dulbecco R
出版信息
Proc Natl Acad Sci U S A. 1972 Jan;69(1):143-6. doi: 10.1073/pnas.69.1.143.
Abstract
Nuclei from secondary mouse-embryo cells contain an activity capable of untwisting closed-circular DNAs containing either negative or positive superhelical turns. The activity has no apparent effect on a closed-circular DNA containing no superhelical turns, and is not due to the combined action of an endonuclease and polynucleotide ligase. The enzyme apparently acts by introducing a single-strand nick into the DNA, forming a DNA-enzyme complex that allows the strands to rotate relative to the helix axis before reversing the reaction and sealing the break. The enzyme might possibly serve as a swivel during DNA replication.
摘要
来自次级小鼠胚胎细胞的细胞核含有一种活性物质，它能够解开含有负超螺旋或正超螺旋的闭环DNA。这种活性对不含超螺旋的闭环DNA没有明显影响，并且不是由核酸内切酶和多核苷酸连接酶的联合作用引起的。该酶显然是通过在DNA中引入一个单链切口来起作用的，形成一种DNA - 酶复合物，该复合物允许链相对于螺旋轴旋转，然后逆转反应并封闭切口。该酶可能在DNA复制过程中起到旋转酶的作用。
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