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1
An activity from mammalian cells that untwists superhelical DNA--a possible swivel for DNA replication (polyoma-ethidium bromide-mouse-embryo cells-dye binding assay).一种来自哺乳动物细胞的能解开超螺旋DNA的活性物质——一种可能用于DNA复制的旋转酶（多瘤病毒-溴化乙锭-小鼠胚胎细胞-染料结合测定）
Proc Natl Acad Sci U S A. 1972 Jan;69(1):143-6. doi: 10.1073/pnas.69.1.143.
2
Is a specific protein responsible for the supercoiling of polyoma DNA?是否有一种特定的蛋白质负责多瘤病毒DNA的超螺旋化？
Nature. 1972 Jan 14;235(5333):105-7. doi: 10.1038/235105a0.
3
Attempts to find a specific intranuclear location for replication polyoma virus DNA.寻找多瘤病毒DNA复制的特定核内位置的尝试。
Can J Biochem. 1973 Sep;51(9):1225-8. doi: 10.1139/o73-162.
4
Protein and messenger RNA requirements for superhelicity of polyoma virus DNA.多瘤病毒DNA超螺旋形成所需的蛋白质和信使RNA条件
Nat New Biol. 1973 Apr 18;242(120):202-4. doi: 10.1038/newbio242202a0.
5
Isolation and partial characterisation of the relaxation protein from nuclei of cultured mouse and human cells.培养的小鼠和人细胞核中松弛蛋白的分离及部分特性鉴定
Eur J Biochem. 1975 Jun 16;55(1):79-93. doi: 10.1111/j.1432-1033.1975.tb02140.x.
6
Isolation of a polyoma-nucleoprotein complex from infected mouse-cell cultures.从感染的小鼠细胞培养物中分离多瘤病毒核蛋白复合物。
Proc Natl Acad Sci U S A. 1971 May;68(5):1032-6. doi: 10.1073/pnas.68.5.1032.
7
Evidence for RNA-linked nascent strands in polyoma virus DNA replication.多瘤病毒DNA复制中RNA连接新生链的证据。
Biochem Biophys Res Commun. 1973 Aug 6;53(3):818-23. doi: 10.1016/0006-291x(73)90166-6.
8
Closed circular DNAs with tandem repeats of a sequence from polyoma virus.含有多瘤病毒序列串联重复的闭环DNA。
Virology. 1974 Sep;61(1):140-55. doi: 10.1016/0042-6822(74)90249-9.
9
DNA synthesis in polyoma virus infection. II. Relationship between viral DNA replication and initiation of cellular DNA replicons.多瘤病毒感染中的DNA合成。II. 病毒DNA复制与细胞DNA复制子起始之间的关系。
J Mol Biol. 1973 Jun 25;78(1):237-45. doi: 10.1016/0022-2836(73)90441-5.
10
The cytoplasmic DNAs of cultured human cells. Effects of ethidium bromide on their replication and maintenance.培养的人类细胞的细胞质DNA。溴化乙锭对其复制和维持的影响。
Eur J Biochem. 1972 Oct 17;30(1):53-9. doi: 10.1111/j.1432-1033.1972.tb02071.x.

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Unraveling the Role of Topoisomerase 3β (TOP3B) in mRNA Translation and Human Disease.解析拓扑异构酶3β（TOP3B）在mRNA翻译及人类疾病中的作用
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Mechanisms controlling replication fork stalling and collapse at topoisomerase 1 cleavage complexes.调控拓扑异构酶 1 酶切复合物复制叉停滞和崩溃的机制。
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Topoisomerases in Immune Cell Development and Function.免疫细胞发育和功能中的拓扑异构酶。
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Natural Compounds as Therapeutic Agents: The Case of Human Topoisomerase IB.天然化合物作为治疗剂：以人类拓扑异构酶 IB 为例。
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DNA topoisomerases: Advances in understanding of cellular roles and multi-protein complexes via structure-function analysis.DNA 拓扑异构酶：通过结构-功能分析深入了解细胞作用和多蛋白复合物。
Bioessays. 2021 Apr;43(4):e2000286. doi: 10.1002/bies.202000286. Epub 2021 Jan 22.

本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Purification of polyoma virus.多瘤病毒的纯化
Virology. 1963 Feb;19:158-68. doi: 10.1016/0042-6822(63)90005-9.
3
Studies on cells rendered neoplastic by polyoma virus: the problem of the presence of virus-related materials.关于由多瘤病毒诱发肿瘤的细胞的研究：病毒相关物质的存在问题。
Virology. 1962 Jan;16:41-51. doi: 10.1016/0042-6822(62)90200-3.
4
A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.用于比色法测定脱氧核糖核酸的二苯胺反应的条件及机制研究。
Biochem J. 1956 Feb;62(2):315-23. doi: 10.1042/bj0620315.
5
A fluorescent complex between ethidium bromide and nucleic acids. Physical-chemical characterization.溴化乙锭与核酸之间的荧光复合物。物理化学特性
J Mol Biol. 1967 Jul 14;27(1):87-106. doi: 10.1016/0022-2836(67)90353-1.
6
Complex formation between ethidium bromide and nucleic acids.溴化乙锭与核酸之间的复合物形成。
J Mol Biol. 1965 Aug;13(1):269-82. doi: 10.1016/s0022-2836(65)80096-1.
7
Superhelix density heterogeneity of intracellular simian virus 40 deoxyribonucleic acid.细胞内猿猴病毒40脱氧核糖核酸的超螺旋密度异质性
J Virol. 1971 Jan;7(1):1-7. doi: 10.1128/JVI.7.1.1-7.1971.
8
Interaction between DNA and an Escherichia coli protein omega.DNA与大肠杆菌蛋白ω之间的相互作用。
J Mol Biol. 1971 Feb 14;55(3):523-33. doi: 10.1016/0022-2836(71)90334-2.
9
Variation of the average rotation angle of the DNA helix and the superhelical turns of covalently closed cyclic lambda DNA.共价闭合环状λ噬菌体DNA的DNA螺旋平均旋转角度和超螺旋匝数的变化
J Mol Biol. 1969 Jul 14;43(1):25-39. doi: 10.1016/0022-2836(69)90076-x.
10
Replicating SV40 molecules containing closed circular template DNA strands.复制含有闭环模板DNA链的猴病毒40（SV40）分子。
Nat New Biol. 1971 Sep 15;233(37):72-5. doi: 10.1038/newbio233072a0.

一种来自哺乳动物细胞的能解开超螺旋DNA的活性物质——一种可能用于DNA复制的旋转酶（多瘤病毒-溴化乙锭-小鼠胚胎细胞-染料结合测定）

An activity from mammalian cells that untwists superhelical DNA--a possible swivel for DNA replication (polyoma-ethidium bromide-mouse-embryo cells-dye binding assay).

作者信息

Champoux J J, Dulbecco R

出版信息

Proc Natl Acad Sci U S A. 1972 Jan;69(1):143-6. doi: 10.1073/pnas.69.1.143.

DOI:10.1073/pnas.69.1.143
PMID:4333036
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC427563/
Abstract

Nuclei from secondary mouse-embryo cells contain an activity capable of untwisting closed-circular DNAs containing either negative or positive superhelical turns. The activity has no apparent effect on a closed-circular DNA containing no superhelical turns, and is not due to the combined action of an endonuclease and polynucleotide ligase. The enzyme apparently acts by introducing a single-strand nick into the DNA, forming a DNA-enzyme complex that allows the strands to rotate relative to the helix axis before reversing the reaction and sealing the break. The enzyme might possibly serve as a swivel during DNA replication.

摘要

来自次级小鼠胚胎细胞的细胞核含有一种活性物质，它能够解开含有负超螺旋或正超螺旋的闭环DNA。这种活性对不含超螺旋的闭环DNA没有明显影响，并且不是由核酸内切酶和多核苷酸连接酶的联合作用引起的。该酶显然是通过在DNA中引入一个单链切口来起作用的，形成一种DNA - 酶复合物，该复合物允许链相对于螺旋轴旋转，然后逆转反应并封闭切口。该酶可能在DNA复制过程中起到旋转酶的作用。