嗜血杆菌内切脱氧核糖核酸酶对生物活性脱氧核糖核酸的作用。
Action of haemophilus endodeoxyribonuclease on biologically active deoxyribonucleic acid.
作者信息
Gromkova R, Goodgal S H
出版信息
J Bacteriol. 1972 Mar;109(3):987-92. doi: 10.1128/jb.109.3.987-992.1972.
Abstract
An enzyme similar to that described by Smith and Wilcox (15) for Haemophilus influenzae which attacks foreign deoxyribonucleic acid (DNA) but not its own has been isolated and purified from H. parainfluenzae. The enzyme degrades foreign DNA to limited sizes and can destroy the transforming activity of H. influenzae and Bacillus subtilis DNA. The enzyme can also destroy the biological activity of H. influenzae phage and prophage DNA. On the other hand, the H. influenzae endodeoxyribonuclease can destroy the transforming activity of H. parainfluenzae DNA but not its own DNA. It also attacks B. subtilis DNA and its transforming activity.
摘要
已从副流感嗜血杆菌中分离并纯化出一种类似于史密斯和威尔科克斯（15）所描述的流感嗜血杆菌的酶，该酶可攻击外来脱氧核糖核酸（DNA），但不攻击自身的DNA。这种酶将外来DNA降解为有限大小，并能破坏流感嗜血杆菌和枯草芽孢杆菌DNA的转化活性。该酶还能破坏流感嗜血杆菌噬菌体和原噬菌体DNA的生物活性。另一方面，流感嗜血杆菌内切脱氧核糖核酸酶可破坏副流感嗜血杆菌DNA的转化活性，但不破坏其自身DNA。它还攻击枯草芽孢杆菌DNA及其转化活性。
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