Nowinski R C, Fleissner E, Sarkar N H, Aoki T
J Virol. 1972 Feb;9(2):359-66. doi: 10.1128/JVI.9.2.359-366.1972.
Proteins of leukemia and sarcoma viruses from the chicken, mouse, hamster, and cat were analyzed by gel filtration in guanidine hydrochloride. The mammalian viruses were found to contain six major proteins, whereas the avian viruses contained seven proteins. Proteins of viruses from different mammalian species had the same molecular weights which also closely resembled the molecular weights of the six equivalent avian viral proteins. These results defined a basic similarity in protein composition for the C-type viruses of avian and mammalian origin. The two glyco-proteins of murine leukemia virus were identified immunologically as constituents of the viral membrane. Antisera prepared against other proteins distinguished individual internal viral antigens in immunodiffusion tests and also reacted in immunofluorescence with cytoplasmic components of infected cells. Antisera which reacted with the major internal viral proteins did not contain antibodies inhibitory to the viral reverse transcriptase.
通过在盐酸胍中进行凝胶过滤，对来自鸡、小鼠、仓鼠和猫的白血病病毒和肉瘤病毒的蛋白质进行了分析。发现哺乳动物病毒含有六种主要蛋白质，而禽病毒含有七种蛋白质。来自不同哺乳动物物种的病毒蛋白质具有相同的分子量，这些分子量也与六种等效禽病毒蛋白质的分子量非常相似。这些结果确定了禽源和哺乳动物源C型病毒在蛋白质组成上的基本相似性。小鼠白血病病毒的两种糖蛋白在免疫学上被鉴定为病毒膜的成分。针对其他蛋白质制备的抗血清在免疫扩散试验中可区分个体内部病毒抗原，并且在免疫荧光中也与感染细胞的细胞质成分发生反应。与主要内部病毒蛋白质发生反应的抗血清不含有抑制病毒逆转录酶的抗体。
