Velicer L F, Graves D C
J Virol. 1974 Sep;14(3):700-3. doi: 10.1128/JVI.14.3.700-703.1974.
Radioactive labeling of proteins from guanidine-hydrochloride disrupted feline leukemia virus was done in vitro by attaching [(3)H]methyl groups to protein amino groups with reductive alkylation. When in vitro labeled disrupted virus was analyzed by gel filtration in the presence of 6 M guanidine-hydrochloride the expected six major and one minor protein peaks were detected. When the same preparation was analyzed by immunodiffusion, the three antigens tested were found to have retained their antigenic activity.
通过用还原烷基化法将[³H]甲基连接到蛋白质氨基上，在体外对来自盐酸胍裂解的猫白血病病毒的蛋白质进行放射性标记。当在6M盐酸胍存在下通过凝胶过滤分析体外标记的裂解病毒时，检测到预期的六个主要蛋白峰和一个次要蛋白峰。当通过免疫扩散分析相同的制剂时，发现所测试的三种抗原保留了它们的抗原活性。
