苯丙胺对通过大鼠离体颈上神经节的重复性冲动传递的影响。

Effect of amphetamine on the transmission of repetitive impulses through the isolated superior cervical ganglion of the rat.

Downing O A

Br J Pharmacol. 1972 Jan;44(1):71-9. doi: 10.1111/j.1476-5381.1972.tb07239.x.

Abstract

The cervical sympathetic trunk of the isolated rat superior cervical ganglion was stimulated with short bursts of repetitive pulses. At room temperature with rates of stimulation of 4 Hz and above, the ganglionic action potentials were reduced in size.2. Amphetamine (2.7 x 10(-5)M), which caused some depression of transmission during stimulation at 0.1 Hz, caused a partial reversal of the depression of transmission occurring with rates of stimulation above 4 Hz.3. This action of amphetamine was mimicked by adrenaline (3 x 10(-5)M) and noradrenaline (9.6 x 10(-5)M) but not by isoprenaline (1.8 x 10(-5)M) and was unaffected by propranolol (1.4 x 10(-5)M) but was abolished by prior application of phenoxybenzamine (5.8 x 10(-6)M). Furthermore, this action of amphetamine was unaltered in ganglia taken from rats pretreated with reserpine (single dose of 6 mg/kg, 16 h before dissection).4. Amphetamine had no effect on the surface potentials of the ganglion or on changes in these potentials produced by concentrations of carbachol (5. x 10(-6)M to 5.5 x 10(-3)M).5. It is concluded that amphetamine has a direct action on alpha-adrenoceptors situated at presynaptic sites.

摘要

对分离出的大鼠颈上神经节的颈交感干施加短串重复脉冲刺激。在室温下，刺激频率为4Hz及以上时，神经节动作电位的幅度减小。
苯丙胺（2.7×10⁻⁵M）在0.1Hz刺激时会引起一定程度的传递抑制，而在刺激频率高于4Hz时能部分逆转传递抑制。
肾上腺素（3×10⁻⁵M）和去甲肾上腺素（9.6×10⁻⁵M）可模拟苯丙胺的这种作用，而异丙肾上腺素（1.8×10⁻⁵M）则不能，普萘洛尔（1.4×10⁻⁵M）对其无影响，但预先应用酚苄明（5.8×10⁻⁶M）可消除该作用。此外，在用利血平预处理（解剖前16小时单剂量6mg/kg）的大鼠取出的神经节中，苯丙胺的这种作用未改变。
苯丙胺对神经节的表面电位或由卡巴胆碱（5×10⁻⁶M至5.5×10⁻³M）浓度引起的这些电位变化没有影响。
得出结论：苯丙胺对位于突触前部位的α-肾上腺素能受体有直接作用。