节前神经末梢中乙酰胆碱储存的电生理分析。
An electrophysiological analysis of the storage of acetylcholine in preganglionic nerve terminals.
作者信息
Bennett M R, McLachlan E M
出版信息
J Physiol. 1972 Mar;221(3):657-68. doi: 10.1113/jphysiol.1972.sp009774.
Abstract
- An electrophysiological analysis has been made of the storage of acetylcholine (ACh) in the preganglionic nerve terminals of the isolated superior cervical ganglion of the guinea-pig. The mean amplitude of excitatory post-synaptic potentials recorded intracellularly was taken as a measure of the ACh output per impulse from the terminals of a preganglionic axon.2. Prolonged repetitive stimulation of the cervical sympathetic trunk at 10 and 20 Hz in the presence of hemicholinium No. 3 led to an exponential decline of ACh output as the transmitter formed before the beginning of stimulation was depleted.3. The rate of decline of ACh output during stimulation at 20 Hz (tau = 0.83-1.95 min) was about twice as fast as that at 10 Hz (tau = 1.00-6.83 min).4. The results suggest that, during prolonged stimulation, ACh is released from a single store in the preganglionic nerve terminal and that each impulse releases a constant fraction of the store of the transmitter.
摘要
对豚鼠离体颈上神经节节前神经末梢中乙酰胆碱（ACh）的储存进行了电生理分析。细胞内记录的兴奋性突触后电位的平均幅度被用作衡量节前轴突末梢每冲动释放ACh量的指标。
在存在三号半胆碱的情况下，以10赫兹和20赫兹对颈交感干进行长时间重复刺激，随着刺激开始前形成的递质耗尽，ACh释放量呈指数下降。
20赫兹刺激期间ACh释放量的下降速率（τ = 0.83 - 1.95分钟）约为10赫兹刺激时（τ = 1.00 - 6.83分钟）的两倍。
结果表明，在长时间刺激期间，ACh从节前神经末梢的单一储存库中释放，且每个冲动释放递质储存库的恒定比例。
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