病毒和细菌神经氨酸酶对淋巴细胞补体敏感性的比较作用
Comparative effect of viral and bacterial neuraminidase on the complement sensitivity of lymphoid cells.
作者信息
Ray P K, Simmons R L
出版信息
Clin Exp Immunol. 1972 Jan;10(1):139-50.
C3H/HeJ mouse lymphoid cells were exposed to neuraminidase (VCN), neuraminidase (CPN), and influenza virus neuraminidase (IVN). Exposure of mouse lymphoid cells to VCN or CPN in non-cytotoxic concentrations renders such cells susceptible to lysis by specific antibody and rabbit serum complement and by rabbit serum alone. Inactivation of complement eliminates the effect. Exposure of rabbit lymphoid cells to VCN or CPN renders them susceptible to lysis in the absence of antibody when autologous complement is activated within the fluid phase by cobra venom factor. IVN does not sensitize lymphoid cells to complement in these systems. VCN and CPN are known to hydrolyse the 2–3', 2–6' and 2–8' glycosidic linkages between sialic acid and mucopolysaccharides on the cell membrane. IVN hydrolysed only the 2–3' and 2–8' glycosidic linkages. Thus, destruction of the 2–6' glycosidic linkage renders such cells highly susceptible to lysis by complement. Quantitation of sialic acid released from cells treated with VCN or IVN demonstrates that most of th esialic acid is bound to the cell surface by the 2–6' glycosidic linkage.
将C3H/HeJ小鼠淋巴细胞暴露于神经氨酸酶（VCN）、神经氨酸酶（CPN）和流感病毒神经氨酸酶（IVN）。以无细胞毒性的浓度将小鼠淋巴细胞暴露于VCN或CPN，会使这些细胞易于被特异性抗体和兔血清补体以及仅兔血清裂解。补体的失活消除了这种效应。将兔淋巴细胞暴露于VCN或CPN，当通过眼镜蛇毒因子在液相中激活自体补体时，它们在没有抗体的情况下易于被裂解。在这些系统中，IVN不会使淋巴细胞对补体敏感。已知VCN和CPN可水解细胞膜上唾液酸与粘多糖之间的2–3'、2–6'和2–8'糖苷键。IVN仅水解2–3'和2–8'糖苷键。因此，2–6'糖苷键的破坏使这些细胞极易被补体裂解。对用VCN或IVN处理的细胞释放的唾液酸进行定量分析表明，大部分唾液酸通过2–6'糖苷键与细胞表面结合。
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