Stoff J S, Handler J S, Orloff J
Proc Natl Acad Sci U S A. 1972 Apr;69(4):805-8. doi: 10.1073/pnas.69.4.805.
Vasopressin and theophylline both increase the content of adenosine 3':5'-cyclic monophosphate (cAMP) in epithelial cells of the urinary bladder of toads (Bufo marinus). Incubation of the tissue with 0.2 muM aldosterone markedly increases this response to the two agents; incubation for a similar time without steroid reduces the response. The permeability responses (sodium transport and water flow) of the intact tissue to vasopressin, theophylline, and exogenous cAMP are also considerably greater in bladders incubated with aldosterone than without. The results are interpreted as indicating that the foregoing permissive effects of aldosterone on the permeability responses to vasopressin and theophylline are mediated by a steroid-dependent increase in the accumulation of cAMP in the pertinent epithelial cells, probably as a consequence of a diminution in the rate of degradation of the intracellular nucleotide.
血管加压素和茶碱均可增加蟾蜍（海蟾蜍）膀胱上皮细胞中3':5'-环磷酸腺苷（cAMP）的含量。用0.2μM醛固酮孵育组织可显著增强对这两种药物的反应；在无类固醇的情况下进行类似时间的孵育则会降低反应。与未用醛固酮孵育的膀胱相比，用醛固酮孵育的完整组织对血管加压素、茶碱和外源性cAMP的通透性反应（钠转运和水流）也明显更强。这些结果被解释为表明，醛固酮对血管加压素和茶碱通透性反应的上述允许作用是由相关上皮细胞中cAMP积累的类固醇依赖性增加介导的，这可能是细胞内核苷酸降解速率降低的结果。
