Pietta P G, Agnellini D, Pace M
J Pharm Sci. 1979 Dec;68(12):1565-6. doi: 10.1002/jps.2600681231.
A colorimetric method based on the interaction between the chloramphenicol degradation product 1-(4'-nitrophenyl)-2-aminopropane-1,3-diol and the 2,4,6-trinitrobenzenesulfonic acid reagent was developed. Analytical solutions were reacted with the reagent at pH 9.1 for 20 min at room temperature, and the resulting color was measured at 340 nm. A linear relationship between absorbance and concentration occurred within the 5--25-micrograms/ml range under the conditions studied. Replicate analyses were in good agreement. An average recovery of 99.4 +/- 0.4% was obtained for the synthetic mixtures.
开发了一种基于氯霉素降解产物1-(4'-硝基苯基)-2-氨基丙烷-1,3-二醇与2,4,6-三硝基苯磺酸试剂之间相互作用的比色法。分析溶液在室温下于pH 9.1与该试剂反应20分钟，然后在340nm处测量所得颜色。在所研究的条件下，吸光度与浓度在5-25微克/毫升范围内呈线性关系。重复分析结果吻合良好。合成混合物的平均回收率为99.4±0.4%。
