细胞结合干扰素的回收
Recovery of cell-bound interferon.
作者信息
Stewart W E, De Clercq E, De Somer P
出版信息
J Virol. 1972 Oct;10(4):707-12. doi: 10.1128/JVI.10.4.707-712.1972.
Interferon could be recovered from homologous cells to which it was applied but could not be recovered from heterologous cells. The amount of interferon that could be recovered from cells corresponded to the sensitivity of the cells to the antiviral activity of the interferon: mouse embryo fibroblasts, which were 5 to 10 times as sensitive as L-929 cells to interferon, bound 5 to 10 times more interferon than the latter, whereas Lpa cells, which were only one-third as sensitive as L-929 cells to interferon, bound only one-third as much as the latter. The concentration of cell-bound interferon was as much as 150 times the extracellular concentration of interferon applied to the cells. Interferon bound to cells at 4 C with the same efficiency as it did to cells at 37 C, and actinomycin D-treated cells bound interferon as well as normal cells. Even though the total amount of interferon bound to cells was as much as 30% of the amount of interferon applied to them, no loss of antiviral activity was detectable from the medium.
干扰素可以从应用了它的同源细胞中回收，但无法从异源细胞中回收。从细胞中回收的干扰素量与细胞对干扰素抗病毒活性的敏感性相对应：小鼠胚胎成纤维细胞对干扰素的敏感性是L-929细胞的5至10倍，其结合的干扰素比后者多5至10倍；而Lpa细胞对干扰素的敏感性仅为L-929细胞的三分之一，其结合的干扰素量也仅为后者的三分之一。细胞结合的干扰素浓度高达施加到细胞上的细胞外干扰素浓度的150倍。干扰素在4℃与细胞结合的效率与在37℃时相同，经放线菌素D处理的细胞与正常细胞一样能结合干扰素。尽管结合到细胞上的干扰素总量高达施加到细胞上的干扰素量的30%，但在培养基中未检测到抗病毒活性的损失。
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