丹麦猫细小核糖核酸病毒和疱疹病毒的分离与分类
Isolation and classification of feline picornavirus and herpesvirus in Denmark.
作者信息
Flagstad A
出版信息
Acta Vet Scand. 1972;13(4):462-71. doi: 10.1186/BF03547152.
Seventeen viral isolates cytopathic for kitten kidney cells were isolated from 23 cats with symptoms of the respiratory disease feline viral rhinotracheitis or feline influenza. Five of these are classified tentatively as calicivirus, a member of the Picornavirus group, and 12 have the properties of the herpesvirus. Classification is based on the cytopathic effect in cell cultures and physico-chemical characteristics. The five isolates which are classified tentatively as calicivirus produced a quick cytopathic effect without formation of intranuclear inclusion bodies. The isolates were resistant to chloroform, were not inhibited by IDU, were labile at pH 4 and were not stabilized against thermal inactivation by molar MgCl. The 12 isolates which seem to belong to the herpesvirus produced intranuclear inclusion bodies in cell cultures. They were sensitive to chloroform, and multiplication was inhibited by IDU. Antisera were produced by inoculating rabbits with calicivirus and cats with herpesvirus. The five isolates classified tentatively as calicivirus belong in one serotype, and the 12 isolates which seem to belong to the herpesvirus also belong in one serotype.
从23只患有呼吸道疾病猫病毒性鼻气管炎或猫流感症状的猫中分离出17株对猫肾细胞具有细胞病变效应的病毒分离株。其中5株暂定为杯状病毒，属于小核糖核酸病毒组的一员，12株具有疱疹病毒的特性。分类基于细胞培养中的细胞病变效应和物理化学特征。暂定为杯状病毒的5株分离株产生快速细胞病变效应，不形成核内包涵体。这些分离株对氯仿有抗性，不受碘苷抑制，在pH 4时不稳定，且不被摩尔氯化镁稳定以抵抗热灭活。似乎属于疱疹病毒的12株分离株在细胞培养中产生核内包涵体。它们对氯仿敏感，繁殖受到碘苷抑制。通过用杯状病毒接种兔子和用疱疹病毒接种猫来制备抗血清。暂定为杯状病毒的5株分离株属于一个血清型，似乎属于疱疹病毒的12株分离株也属于一个血清型。
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