Havel R J, Kane J P, Kashyap M L
J Clin Invest. 1973 Jan;52(1):32-8. doi: 10.1172/JCI107171.
Apolipoproteins of the "C" group in human blood plasma, which contain the activator of the lipoprotein lipase-substrate interaction, were found to be transferred specifically from serum to phospholipid-stabilized fat emulsion. Content and distribution of apoprotein activator were measured in healthy men in the postabsorptive state and 4 h after ingestion of meals containing 100 g fat. Content of activator protein in whole serum did not change after ingestion of the fat-rich meals but that contained in triglyceride-rich lipoproteins of density (d) <1.006 approximately doubled whereas that of high density lipoproteins fell by half. The increased activator content of triglyceride-rich lipoproteins was virtually confined to chylomicrons and its concentration in chylomicron apoprotein was substantially greater than that in very low density lipoproteins. This difference could be ascribed largely to a higher content of C apoproteins in chylomicron protein since both the concentration of C apoproteins and of apoprotein activator were directly proportional to particle diameter while the pattern of fast-migrating C apoproteins in polyacrylamide gels was similar among chylomicrons and subfractions of very low density lipoproteins. Apparent concentration of activator protein was much greater in the high density lipoprotein subfraction of d 1.063-1.125 than in the subfraction of d 1.125-1.21. In the subfraction of d 1.063-1.125, the concentration of activator protein and of fast-migrating C apoproteins in polyacrylamide gels decreased after the fat-rich meal. Concentration of phospholipids in this fraction increased gradually to a peak 43% above the basal value 6 h after the meal. The results obtained demonstrate that high density lipoproteins contribute certain functionally important polar constituents to chylomicrons during alimentary lipemia in man and suggest that they also receive surface constituents from chylomicrons during the course of their metabolism.
人血浆中含有脂蛋白脂肪酶 - 底物相互作用激活剂的“C”组载脂蛋白，被发现可特异性地从血清转移至磷脂稳定的脂肪乳剂中。在健康男性的空腹状态以及摄入含100克脂肪的餐后4小时，对载脂蛋白激活剂的含量和分布进行了测定。摄入富含脂肪的餐后，全血清中激活蛋白的含量未发生变化，但密度（d）<1.006的富含甘油三酯的脂蛋白中所含的激活蛋白含量大约增加了一倍，而高密度脂蛋白中的激活蛋白含量则下降了一半。富含甘油三酯的脂蛋白中激活剂含量的增加实际上仅限于乳糜微粒，其在乳糜微粒载脂蛋白中的浓度显著高于极低密度脂蛋白中的浓度。这种差异很大程度上可归因于乳糜微粒蛋白中C载脂蛋白的含量较高，因为C载脂蛋白和载脂蛋白激活剂的浓度均与颗粒直径成正比，而乳糜微粒和极低密度脂蛋白亚组分在聚丙烯酰胺凝胶中快速迁移的C载脂蛋白模式相似。在密度为1.063 - 1.125的高密度脂蛋白亚组分中，激活蛋白的表观浓度远高于密度为1.125 - 1.21的亚组分。在密度为1.063 - 1.125的亚组分中，富含脂肪的餐后，聚丙烯酰胺凝胶中激活蛋白和快速迁移的C载脂蛋白的浓度降低。该组分中磷脂的浓度在餐后6小时逐渐增加至比基础值高43%的峰值。所获得的结果表明，在人类消化性脂血症期间，高密度脂蛋白向乳糜微粒贡献了某些功能上重要的极性成分，并表明它们在代谢过程中也从乳糜微粒接收表面成分。
