由常见等位基因DIA13、DIA23和DIA33所决定的人类心肌黄酶（DIA3）同工酶的生化特性比较。

A comparison of the biochemical properties of the human diaphorase (DIA3) isozymes determined by the common alleles DIA13, DIA23 and DIA33.

作者信息

Edwards Y H, Potter J E, Hopkinson D A

出版信息

Ann Hum Genet. 1979 Jan;42(3):293-302. doi: 10.1111/j.1469-1809.1979.tb00663.x.

DOI:10.1111/j.1469-1809.1979.tb00663.x

Abstract

(1) Various buffer systems for the starch gel electrophoresis of human diaphorase isozymes have been explored. Electrophoresis in a Tris/Borate system at pH 8.6 which includes 70 micron NADH in the gel and cathodal electrode buffers, provides good resolution of the six DIA3 phenotypes previously resolved by isoelectric focusing. (2) The variant genes DIA13, DIA23 and DIA33 occur with frequencies of about 0.76, 0.23 and 0.01 respectively in the English population. (3) The isozymes determined by the least common gene, DIA33, are markedly different from the isozymes determined by DIA13 and DIA23 in their relatively low heat stability, high affinity for Blue Sepharose and slow anodal electrophoretic mobility in buffer systems containing borate. The DIA3 1 and DIA3 2 isozymes are similar to one another in these characteristics.

摘要

(1) 已对用于人黄递酶同工酶淀粉凝胶电泳的各种缓冲系统进行了探索。在pH 8.6的Tris/硼酸盐系统中进行电泳，该系统在凝胶和阴极电极缓冲液中包含70微米的NADH，能很好地分辨出先前通过等电聚焦分辨出的六种DIA3表型。(2) 变异基因DIA13、DIA23和DIA33在英国人群中的出现频率分别约为0.76、0.23和0.01。(3) 由最不常见的基因DIA33所决定的同工酶，在相对较低的热稳定性、对蓝色琼脂糖的高亲和力以及在含硼酸盐的缓冲系统中缓慢的阳极电泳迁移率方面，与由DIA13和DIA23所决定的同工酶明显不同。DIA3 1和DIA3 2同工酶在这些特性上彼此相似。