一种通过等电聚焦对人类精液中的人类黄递酶基因座3进行表型分析的方法。
A method of human diaphorase locus 3 phenotyping in human semen by isoelectric focusing.
作者信息
Pawlowski R
机构信息
Institute of Forensic Medicine, Medical Academy Gdańsk, Poland.
出版信息
Forensic Sci Int. 1989 Jul;42(1-2):131-4. doi: 10.1016/0379-0738(89)90205-3.
PMID:2759518
Abstract
A rapid method for the separation of diaphorase (DIA 3) isozymes in the pH range 6-8 is presented. Isoelectric focusing (IEF) of DIA 3 on 0.2 mm thick polyacrylamide gels took 60 min at 5 degrees C. Separated bands are sharp and common phenotypes are readily distinguishable. The method combines high resolution, speed, reproducibility and reagent economy with simplicity.
摘要
本文介绍了一种在pH值6 - 8范围内快速分离黄递酶（DIA 3）同工酶的方法。在5℃条件下，将DIA 3在0.2毫米厚的聚丙烯酰胺凝胶上进行等电聚焦（IEF）需60分钟。分离出的条带清晰，常见表型易于区分。该方法将高分辨率、速度、可重复性、试剂经济性与简便性相结合。
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