苯二氮䓬受体：37摄氏度下组织预孵育的影响

Benzodiazepine receptors: effect of tissue preincubation at 37 degrees C.

作者信息

Speth R C, Wastek G J, Reisine T D, Yamamura H I

出版信息

Neurosci Lett. 1979 Aug;13(3):243-7. doi: 10.1016/0304-3940(79)91501-5.

DOI:10.1016/0304-3940(79)91501-5

PMID:43491

Abstract

[3H]Flunitrazepam binding to rat brain homogenates was assayed in two ways: by 37-0 degrees C incubations or 0 degrees C only incubations. The affinity of brain benzodiazepine receptors for [3H]flunitrazepam was significantly greater in 37-0 degrees C incubated samples than in 0 degrees C incubated samples, while there was no change in the number of benzodiazepine receptors. The possible mechanisms for this enhanced binding affinity as well as the methodological implications for this observation are discussed.

摘要

[3H]氟硝西泮与大鼠脑匀浆的结合通过两种方式进行测定：在37℃孵育或仅在0℃孵育。与0℃孵育的样品相比，在37℃孵育的样品中脑苯二氮䓬受体对[3H]氟硝西泮的亲和力显著更高，而苯二氮䓬受体的数量没有变化。讨论了这种结合亲和力增强的可能机制以及该观察结果的方法学意义。

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