Millecchia L L, Rudzinska M A
J Cell Biol. 1970 Sep;46(3):553-63. doi: 10.1083/jcb.46.3.553.
Basal body replication and ciliogenesis in Tokophrya infusionum were studied in synchronized cultures. Basal body replication occurs during the 1st hr of reproduction, which in Tokophrya is by internal budding. The number of basal bodies increases from about 20 to over 300 within this period. New basal bodies develop in association with mature basal bodies; they are formed at right angles to the mature basal body as short "probasal" bodies, which elongate, slant upward, become parallel to the mature basal body, and elongate to the mature size. Ciliogenesis occurs only during reproduction; the nonreproducing adult is not ciliated, and has only 18-25 barren basal bodies. Cilia first appear as short bulges above the basal body. The axonemal structure is incomplete at first, with one or both central microtubules absent, and occasionally the B fibers of the outer doublets are missing. Several accessory fibers are associated with the basal bodies, both in the adult and during reproduction. One of the fibers appears only after the cilia have sprouted. The scheme of basal body replication and ciliogenesis in Tokophrya is compared to that reported in other organisms, and the role of the accessory fibers is discussed.
在同步培养物中研究了点滴钟形虫的基体复制和纤毛发生。基体复制发生在繁殖的第1小时，在点滴钟形虫中是通过内芽生殖进行的。在此期间，基体数量从约20个增加到300多个。新的基体与成熟基体相关联发育；它们以短的“原基体”形式与成熟基体成直角形成，这些原基体伸长、向上倾斜、与成熟基体平行，并伸长到成熟大小。纤毛发生仅在繁殖期间发生；不繁殖的成虫没有纤毛，只有18 - 25个不育基体。纤毛最初表现为基体上方的短凸起。轴丝结构最初不完整，缺少一条或两条中央微管，偶尔外双联体的B纤维也缺失。在成虫和繁殖期间，有几根辅助纤维与基体相关联。其中一根纤维仅在纤毛长出后出现。将点滴钟形虫的基体复制和纤毛发生模式与其他生物体中报道的模式进行了比较，并讨论了辅助纤维的作用。
