Sessoms A H, Huskey R J
Proc Natl Acad Sci U S A. 1973 May;70(5):1335-8. doi: 10.1073/pnas.70.5.1335.
Morphogenetic mutants of the colonial green alga, Volvox carteri f. nagariensis, were induced by chemical mutagenesis. The 68 independent mutants are classified into 12 readily identifiable phenotypes affecting various stages of asexual development. Nine of the mutants are temperature sensitive with normal development at 25 degrees and mutant development occurring at 35 degrees . Some mutant genes appear to be involved in the regulation of differentiation or the stability of the differentiated state. Other mutations occur in genes apparently responsible for structural components of dividing cells or adult colonies. Two mutants affect different aspects of the posterior-anterior polarization of the mature colony. One mutation affects a gene which acts very early in colonial development, but affects the appearance of the mature colony. The mutants isolated demonstrate the feasibility of using Volvox to study the genetic control of early steps in embryogenesis.
通过化学诱变诱导了群居绿藻莱茵衣藻的形态发生突变体。这68个独立突变体被分为12种易于识别的表型，这些表型影响无性发育的各个阶段。其中9个突变体对温度敏感，在25摄氏度时正常发育，在35摄氏度时出现突变发育。一些突变基因似乎参与了分化的调控或分化状态的稳定性。其他突变发生在显然负责分裂细胞或成熟群体结构成分的基因中。两个突变体影响成熟群体前后极化的不同方面。一个突变影响一个在群体发育早期起作用但影响成熟群体外观的基因。分离出的突变体证明了利用衣藻研究胚胎发生早期步骤的遗传控制的可行性。
