• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Stimulation of eukaryotic DNA-dependent RNA polymerase by protein factors.

作者信息

Lee S C, Dahmus M E

出版信息

Proc Natl Acad Sci U S A. 1973 May;70(5):1383-7. doi: 10.1073/pnas.70.5.1383.

DOI:10.1073/pnas.70.5.1383
PMID:4351175
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC433502/
Abstract

Protein factors that stimulate DNA-dependent RNA polymerase activity in vitro have been purified from Novikoff ascites cells. These factors are not adsorbed by the diethylaminoethylcellulose column used for RNA polymerase purification and appear in the flow-through fraction. They can be fractionated into two classes by chromatography on carboxymethylcellulose. The first peak of activity elutes at 0.1 M NH(4)Cl, is stable to heat treatment of 100 degrees for 5 min, and is designated heat-stable factor; the second peak of activity elutes at 0.3 M NH(4)Cl, is heat labile, and is designated heat-labile factor. Heat-labile factor can be further resolved into two components by chromatography on phosphocellulose. The heat-stable factor and second heat-labile factor stimulate the activity of (Novikoff ascites) RNA polymerase B by several-fold, and appear to function independently. RNA synthesis is stimulated only with native DNA as a template. No stimulation of Escherichia coli RNA polymerase is observed with any of the factors.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f09a/433502/c2aae9e9c242/pnas00068-0100-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f09a/433502/c2aae9e9c242/pnas00068-0100-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f09a/433502/c2aae9e9c242/pnas00068-0100-a.jpg

相似文献

1
Stimulation of eukaryotic DNA-dependent RNA polymerase by protein factors.
Proc Natl Acad Sci U S A. 1973 May;70(5):1383-7. doi: 10.1073/pnas.70.5.1383.
2
DNA-dependent RNA polymerase from Ehrlich ascites tumor cells. 3. Ribonuclease H and elongating activity of stimulatory factor S-II.艾氏腹水瘤细胞的依赖DNA的RNA聚合酶。3. 核糖核酸酶H与刺激因子S-II的延伸活性
J Biochem. 1973 Dec;74(6):1177-82. doi: 10.1093/oxfordjournals.jbchem.a130345.
3
Stimulation of ascites tumor RNA polymerase II by protein kinase.蛋白激酶对腹水肿瘤RNA聚合酶II的刺激作用。
Biochemistry. 1976 May 4;15(9):1821-9. doi: 10.1021/bi00654a006.
4
RNA polymerases from a rat hepatoma. Partial purification and comparison of properties with corresponding liver enzymes.大鼠肝癌组织中的RNA聚合酶。部分纯化及其与相应肝脏酶性质的比较。
Biochim Biophys Acta. 1976 Apr 15;432(1):60-72. doi: 10.1016/0005-2787(76)90041-1.
5
DNA dependent RNA polymerase from Ehrlich ascites tumor cells. II. Factors stimulating the activity of RNA polymerase II.
J Biochem. 1973 Apr;73(4):879-88. doi: 10.1093/oxfordjournals.jbchem.a130150.
6
Two heat-resistant, low molecular weight proteins from Escherichia coli that stimulate DNA-directed RNA synthesis.来自大肠杆菌的两种耐热性低分子量蛋白质，可刺激DNA指导的RNA合成。
Proc Natl Acad Sci U S A. 1972 Dec;69(12):3643-7. doi: 10.1073/pnas.69.12.3643.
7
A comparative study of DNA-dependent RNA polymerases from rat ascites hepatoma cell nuclei and from rat liver nuclei.大鼠腹水肝癌细胞核与大鼠肝细胞核中依赖DNA的RNA聚合酶的比较研究。
Acta Med Okayama. 1975 Dec;29(6):405-12.
8
Ascites tumor ribonucleic acid polymerases. Isolation, purification, and factor stimulation.腹水肿瘤核糖核酸聚合酶。分离、纯化及因子刺激
Biochemistry. 1973 Jul 31;12(16):3064-71. doi: 10.1021/bi00740a019.
9
Guanosine anabolism for biosynthesis of nucleic acids in Novikoff ascites rat tumor cells in culture.培养的诺维科夫腹水大鼠肿瘤细胞中用于核酸生物合成的鸟苷合成代谢。
Cancer Res. 1973 Oct;33(10):2265-72.
10
Purification of DNA-dependent RNA polymerases A and B from ovaries of Xenopus laevis.从非洲爪蟾卵巢中纯化依赖DNA的RNA聚合酶A和B。
Eur J Biochem. 1974 May 2;44(1):115-22. doi: 10.1111/j.1432-1033.1974.tb03463.x.

引用本文的文献

1
DNA-dependent RNA polymerases from Drosophila melanogaster adults: isolation and partial characterization.黑腹果蝇成虫的DNA依赖性RNA聚合酶：分离与部分特性鉴定
Biochem Genet. 1981 Feb;19(1-2):15-30. doi: 10.1007/BF00486134.
2
Stimulatory proteins of RNA polymerase II from Ehrlich ascites tumor cells.艾氏腹水瘤细胞RNA聚合酶II的刺激蛋白
Mol Cell Biochem. 1982 Aug 6;46(3):173-87. doi: 10.1007/BF00239666.
3
Increased frequency of initiation of RNA synthesis due to a protein factor from chicken myeloblastosis nuclei.由于来自鸡成髓细胞瘤细胞核的一种蛋白质因子，RNA合成起始频率增加。

本文引用的文献

1
DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.圆盘电泳。II. 方法及其在人血清蛋白中的应用。
Ann N Y Acad Sci. 1964 Dec 28;121:404-27. doi: 10.1111/j.1749-6632.1964.tb14213.x.
2
DISC ELECTROPHORESIS. I. BACKGROUND AND THEORY.圆盘电泳。一、背景与理论。
Ann N Y Acad Sci. 1964 Dec 28;121:321-49. doi: 10.1111/j.1749-6632.1964.tb14207.x.
3
Deoxyribo ucleic acid-directed synthesis of ribonucleic acid by an enzyme from Escherichia coli.大肠杆菌中一种酶催化的脱氧核糖核酸指导的核糖核酸合成。
Proc Natl Acad Sci U S A. 1975 Aug;72(8):2935-9. doi: 10.1073/pnas.72.8.2935.
4
Effect of non-histone chromosomal proteins on transcription in vitro in sea-urchin.非组蛋白染色体蛋白对海胆体外转录的影响。
Biochem J. 1978 Jul 15;174(1):95-102. doi: 10.1042/bj1740095.
5
Deoxyribonucleic acid-dependent ribonucleic acid polymerases from normal and polyoma-transformed BHK-21/C13 cells.来自正常和多瘤病毒转化的BHK-21/C13细胞的脱氧核糖核酸依赖性核糖核酸聚合酶
Biochem J. 1975 Mar;145(3):509-16. doi: 10.1042/bj1450509.
Proc Natl Acad Sci U S A. 1962 Jan 15;48(1):81-94. doi: 10.1073/pnas.48.1.81.
4
Structural evidence for two alpha-amanitin sensitive RNA polymerases in calf thymus.小牛胸腺中两种对α-鹅膏蕈碱敏感的RNA聚合酶的结构证据。
FEBS Lett. 1971 Jun 24;15(3):169-174. doi: 10.1016/0014-5793(71)80305-8.
5
A new form of mammalian DNA-dependent RNA polymerase and its relationship to the known forms of the enzyme.一种新型的哺乳动物DNA依赖性RNA聚合酶及其与该酶已知形式的关系。
FEBS Lett. 1971 Feb 9;12(6):301-308. doi: 10.1016/0014-5793(71)80001-7.
6
Giant-size rapidly labeled nuclear ribonucleic acid and cytoplasmic messenger ribonucleic acid in immature duck erythrocytes.未成熟鸭红细胞中的巨大快速标记核糖核酸和细胞质信使核糖核酸
J Mol Biol. 1966 Sep;20(1):145-82. doi: 10.1016/0022-2836(66)90123-9.
7
Chromosomal ribonucleic acid of rat ascites cells.大鼠腹水细胞的染色体核糖核酸
Biochemistry. 1969 Apr;8(4):1524-34. doi: 10.1021/bi00832a031.
8
A deoxyribonucleic acid polymerase from Micrococcus luteus (Micrococcus lysodeikticus) isolated on deoxyribonucleic acid-cellulose.从微球菌（溶壁微球菌）中分离得到的一种脱氧核糖核酸聚合酶，该酶是在脱氧核糖核酸 - 纤维素上分离得到的。
J Biol Chem. 1968 Dec 10;243(23):6222-33.
9
A factor from calf thymus stimulating DNA-dependent RNA polymerase isolated from this tissue.一种来自小牛胸腺的因子，可刺激从该组织中分离出的依赖DNA的RNA聚合酶。
Eur J Biochem. 1970 Jun;14(2):270-7. doi: 10.1111/j.1432-1033.1970.tb00286.x.
10
Multiple forms of DNA-dependent RNA polymerase in eukaryotic organisms.真核生物中DNA依赖性RNA聚合酶的多种形式。
Nature. 1969 Oct 18;224(5216):234-7. doi: 10.1038/224234a0.