Slakey L L, Ness G C, Qureshi N, Porter J W
J Lipid Res. 1973 Jul;14(4):485-94.
Homogenates and subcellular fractions of the intimamedia of hog aorta have been prepared and examined for the presence of the enzymes catalyzing the conversion of acetyl CoA to squalene. Enzyme activities effecting the conversion of acetyl CoA to 3-hydroxy-3-methylglutarate (HMG); HMG CoA to mevalonic acid; mevalonic acid to 5-phosphomevalonic acid, 5-pyrophosphomevalonic acid, and isopentenyl pyrophosphate; isopentenyl pyrophosphate to farnesyl pyrophosphate; and farnesyl pyrophosphate to squalene have been demonstrated in these homogenates. The overall conversion of mevalonate to squalene has also been demonstrated with recombined fractions of hog aorta homogenates. Data are also presented that suggest that phosphatases present in the crude homogenates act to cleave farnesyl pyrophosphate to farnesol, and phospho- and pyrophosphomevalonate to mevalonate.
已制备猪主动脉中膜的匀浆和亚细胞组分，并检测其中催化乙酰辅酶A转化为角鲨烯的酶的存在情况。在这些匀浆中已证实存在影响乙酰辅酶A转化为3-羟基-3-甲基戊二酸（HMG）、HMG辅酶A转化为甲羟戊酸、甲羟戊酸转化为5-磷酸甲羟戊酸、5-焦磷酸甲羟戊酸和异戊烯基焦磷酸、异戊烯基焦磷酸转化为法呢基焦磷酸以及法呢基焦磷酸转化为角鲨烯的酶活性。用猪主动脉匀浆的重组组分也已证实甲羟戊酸向角鲨烯的整体转化。还提供了数据表明粗匀浆中存在的磷酸酶可将法呢基焦磷酸裂解为法呢醇，并将磷酸甲羟戊酸和焦磷酸甲羟戊酸裂解为甲羟戊酸。
