人血清蛋白酶结合特性的四线模型
A four-straight-line model for the proteinase-binding characteristics of human blood serum.
作者信息
Topping R M, Seilman S
出版信息
Biochem J. 1979 Feb 1;177(2):493-9. doi: 10.1042/bj1770493.
Abstract
Kinetic evaluation of the capacity of human blood serum to form complexes with bovine trypsin generated partition profiles that may be approximated by a series of four intersecting straight lines. Such profiles are suggested to reflect the binding of trypsin to alpha 2-macroglobulin in a kinetically preferred mode (alpha-sites), followed by a subsidiary mode (beta-sites) and finally to alpha 1-antitrypsin. The form of the profile, in addition to revealing a hitherto unreported proteinase-binding capability of alpha 2-macroglobulin (beta-sites), also indicates that saturation of alpha-sites corresponds to a molar binding ratio of alpha 2-macroglobulin/trypsin of 1:2. Finally the profile provides, for certain pathological states, a clinically valuable characteristic.
摘要
对人血清与牛胰蛋白酶形成复合物能力的动力学评估产生了分配曲线，这些曲线可用一系列四条相交直线近似表示。这种曲线被认为反映了胰蛋白酶以动力学上优先的模式（α位点）与α2-巨球蛋白结合，随后是次要模式（β位点），最后与α1-抗胰蛋白酶结合。该曲线的形式，除了揭示α2-巨球蛋白迄今未报道的蛋白酶结合能力（β位点）外，还表明α位点的饱和对应于α2-巨球蛋白/胰蛋白酶的摩尔结合比为1:2。最后，该曲线为某些病理状态提供了具有临床价值的特征。
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本文引用的文献
1
MACROGLOBULIN FROM HUMAN PLASMA WHICH FORMS AN ENZYMATICALLY ACTIVE COMPOUND WITH TRYPSIN.来自人血浆的巨球蛋白，其与胰蛋白酶形成一种具有酶活性的化合物。
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Protein binding of pancreatic proteolytic enzymes.胰腺蛋白水解酶的蛋白质结合
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The influence of alpha(2)-macroglobulin on the elastolytic and esterolytic activity of elastase.α2-巨球蛋白对弹性蛋白酶的弹性溶解和酯解活性的影响。
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The combining ratio between trypsin and serum alpha-2-macroglobulin.胰蛋白酶与血清α-2-巨球蛋白之间的结合率。
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Amidasic activity of N-episilon-acetyltrypsin free or bound on the alpha-2-macroglobulin.游离或结合于α-2-巨球蛋白上的N-ε-乙酰胰蛋白酶的酰胺酶活性。
Biochem Biophys Res Commun. 1969 Aug 22;36(5):815-22. doi: 10.1016/0006-291x(69)90682-2.
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[Structural and functional properties of complexes between human alpha-2-macroglobulin and trypsin].[人α-2-巨球蛋白与胰蛋白酶复合物的结构和功能特性]
Bull Soc Chim Biol (Paris). 1968;50(11):2003-22.
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[Biochemistry of rabbit alpha 1-macroglobulin. IV. Effect on the esterase activity of trypsin and chymotrypsin].[兔α1-巨球蛋白的生物化学。IV. 对胰蛋白酶和糜蛋白酶酯酶活性的影响]
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