通过离子交换色谱法纯化叶绿体膜二环己基碳二亚胺结合蛋白脂
Purification of the chloroplast-membrane dicyclohexylcarbodi-imide-binding proteolipid by ion-exchange chromatography.
作者信息
Sigrist-Nelson K, Azzi A
出版信息
Biochem J. 1979 Feb 1;177(2):687-92. doi: 10.1042/bj1770687.
Abstract
An efficient, mild and rapid procedure is reported for the separation of the dicyclohexyl-carbodi-imide-binding protein of chloroplast membranes from endogenous lipid components. By the use of ion-exchange chromatography the chloroplast proteolipid can be successfully separated from the major part of chlorophyll and other membrane lipids while being retained in a butan-1-ol milieu.
摘要
报道了一种高效、温和且快速的方法，用于从叶绿体膜的内源性脂质成分中分离二环己基碳二亚胺结合蛋白。通过离子交换色谱法，叶绿体蛋白脂质可以成功地从叶绿素和其他膜脂的主要部分中分离出来，同时保留在丁醇环境中。
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