连二亚硫酸根离子对高铁细胞色素c的还原作用：通过平行相邻和远程途径进行的电子转移
Reduction of ferricytochrome c by dithionite ion: electron transfer by parallel adjacent and remote pathways.
作者信息
Creutz C, Sutin N
出版信息
Proc Natl Acad Sci U S A. 1973 Jun;70(6):1701-3. doi: 10.1073/pnas.70.6.1701.
Abstract
The kinetics of the reduction of horseheart ferricytochrome c by sodium dithionite (phosphate buffer-sodium chloride; pH 6.5, mu = 1.0, 25 degrees ) features two reaction pathways; one with the rate constant k(3) = 1.17 x 10(4) M(-1) sec(-1), the other with the rate constant k(1)k(2)/k(-1) = 6.0 x 10(4) M(-1) sec(-1). These pathways are interpreted in terms of remote attack (possibly by way of the exposed edge of the porphyrin system) and adjacent attack (requiring the opening of the heme crevice). The limiting rate for the adjacent pathway (k(1) = 30 sec(-1)) is in good agreement with the rate of heme-crevice opening of ferricytochrome c determined in other studies. The implication of the adjacent attack pathway to the function of cytochrome c in vivo is discussed.
摘要
连二亚硫酸钠还原马心铁细胞色素c的动力学（磷酸盐缓冲液 - 氯化钠；pH 6.5，离子强度μ = 1.0，25℃）具有两条反应途径；一条的速率常数k(3) = 1.17 x 10⁴ M⁻¹ s⁻¹，另一条的速率常数k(1)k(2)/k(-1) = 6.0 x 10⁴ M⁻¹ s⁻¹。这些途径可通过远程攻击（可能通过卟啉系统暴露的边缘）和相邻攻击（需要血红素裂隙打开）来解释。相邻途径的极限速率（k(1) = 30 s⁻¹）与其他研究中测定的铁细胞色素c血红素裂隙打开速率高度一致。文中讨论了相邻攻击途径对细胞色素c在体内功能的影响。
相似文献
1
Reduction of ferricytochrome c by dithionite ion: electron transfer by parallel adjacent and remote pathways.连二亚硫酸根离子对高铁细胞色素c的还原作用：通过平行相邻和远程途径进行的电子转移
Proc Natl Acad Sci U S A. 1973 Jun;70(6):1701-3. doi: 10.1073/pnas.70.6.1701.
2
Electron transport by C-type cytochromes. I. The reaction of horse heart cytochrome c with anionic reductants.C型细胞色素的电子传递。I. 马心细胞色素c与阴离子还原剂的反应。
Biophys Struct Mech. 1975 Feb 19;1(2):97-111.
3
Model studies for molybdenum enzymes. The reduction of cytochrome c by molybdenum(V)-cysteine complexes.钼酶的模型研究。钼（V）-半胱氨酸复合物对细胞色素c的还原作用。
Biochemistry. 1975 Aug 12;14(16):3626-30. doi: 10.1021/bi00687a017.
4
The mechanism of reduction of cytochrome c as studied by pulse radiolysis.通过脉冲辐解研究细胞色素c还原的机制。
Biochim Biophys Acta. 1975 Feb 17;376(2):285-97. doi: 10.1016/0005-2728(75)90021-3.
5
Kinetics of the reduction of horse heart ferricytochrome c. Ascorbate reduction in the presence and absence of urea.马心铁细胞色素c还原的动力学。在有和没有尿素存在的情况下抗坏血酸的还原作用。
J Biol Chem. 1980 Oct 25;255(20):9666-73.
6
The reduction of porphyrin cytochrome c by hydrated electrons and the subsequent electron transfer reaction from reduced porphyrin cytochrome c to ferricytochrome c.水合电子对卟啉细胞色素c的还原作用以及随后还原型卟啉细胞色素c向高铁细胞色素c的电子转移反应。
Biochim Biophys Acta. 1977 May 11;460(2):290-8. doi: 10.1016/0005-2728(77)90215-8.
7
Reduction of horse heart ferricytochrome c by bovine liver ferrocytochrome b5. Experimental and theoretical analysis.牛肝亚铁细胞色素b5对马心铁细胞色素c的还原作用。实验与理论分析。
Biochemistry. 1991 Apr 16;30(15):3663-74. doi: 10.1021/bi00229a011.
8
Electron transfer to ferricytochrome c: reaction with hydrated electrons and conformational transitions involved.电子向高铁细胞色素c的转移：与水合电子的反应及相关构象转变。
Proc Natl Acad Sci U S A. 1972 Apr;69(4):902-6. doi: 10.1073/pnas.69.4.902.
9
Kinetics and mechanism of electron transfer from dithionite to microsomal cytochrome b5 and to forms of the protein associated with charged and neutral vesicles.连二亚硫酸盐向微粒体细胞色素b5以及与带电和中性囊泡相关的蛋白质形式的电子转移动力学和机制
Biochem J. 1989 Mar 1;258(2):375-80. doi: 10.1042/bj2580375.
10
The oxidation of ferrocytochrome c in nonbinding buffer.非结合缓冲液中亚铁细胞色素c的氧化作用。
Can J Biochem. 1977 Aug;55(8):796-803. doi: 10.1139/o77-118.
引用本文的文献
1
Sulfite Oxidase Activity of Cytochrome c: Role of Hydrogen Peroxide.细胞色素c的亚硫酸盐氧化酶活性：过氧化氢的作用
Biochem Biophys Rep. 2016 Mar 1;5:96-104. doi: 10.1016/j.bbrep.2015.11.025.
2
Redox-dependent stability, protonation, and reactivity of cysteine-bound heme proteins.半胱氨酸结合血红素蛋白的氧化还原依赖性稳定性、质子化和反应性。
Proc Natl Acad Sci U S A. 2014 Jan 21;111(3):E306-15. doi: 10.1073/pnas.1317173111. Epub 2014 Jan 7.
3
Effect of antimicrobial preservatives on partial protein unfolding and aggregation.抗菌防腐剂对部分蛋白质展开和聚集的影响。
J Pharm Sci. 2013 Feb;102(2):365-76. doi: 10.1002/jps.23362. Epub 2012 Nov 20.
4
Interactions of the major metabolite of the cancer chemopreventive drug oltipraz with cytochrome c: a novel pathway for cancer chemoprevention.癌症化学预防药物奥替普拉的主要代谢产物与细胞色素c的相互作用：一种癌症化学预防的新途径。
Free Radic Biol Med. 2007 Oct 1;43(7):1076-85. doi: 10.1016/j.freeradbiomed.2007.06.022. Epub 2007 Jul 6.
5
Kinetics studies of the superoxide-mediated electron transfer reactions between rubredoxin-type proteins and superoxide reductases.铁氧化还原蛋白型蛋白质与超氧化物还原酶之间超氧化物介导的电子转移反应的动力学研究。
J Biol Inorg Chem. 2006 Jun;11(4):433-44. doi: 10.1007/s00775-006-0090-0. Epub 2006 Mar 17.
6
Overexpression and purification of Treponema pallidum rubredoxin; kinetic evidence for a superoxide-mediated electron transfer with the superoxide reductase neelaredoxin.梅毒螺旋体红素氧还蛋白的过表达与纯化；超氧化物介导的与超氧化物还原酶尼尔拉氧还蛋白电子转移的动力学证据
J Biol Inorg Chem. 2004 Oct;9(7):839-49. doi: 10.1007/s00775-004-0584-6. Epub 2004 Aug 20.
7
Structure-function relationship of reduced cytochrome c probed by complete solution structure determination in 30% acetonitrile/water solution.在30%乙腈/水溶液中通过完整溶液结构测定探究还原型细胞色素c的结构-功能关系。
J Biol Inorg Chem. 2003 May;8(5):527-539. doi: 10.1007/s00775-002-0437-0. Epub 2003 Feb 15.
8
Electron transfer in biological systems: an overview.生物系统中的电子转移：概述
Experientia. 1984 Jul 15;40(7):653-75. doi: 10.1007/BF01949719.
9
Nitrogenase of Klebsiella pneumoniae. Kinetics of the dissociation of oxidized iron protein from molybdenum-iron protein: identification of the rate-limiting step for substrate reduction.肺炎克雷伯菌的固氮酶。氧化态铁蛋白与钼铁蛋白解离的动力学：底物还原限速步骤的鉴定。
Biochem J. 1983 Nov 1;215(2):393-403. doi: 10.1042/bj2150393.
10
Reactions of cytochrome c oxidase with sodium dithionite.细胞色素c氧化酶与连二亚硫酸钠的反应。
Biochem J. 1983 Jan 1;209(1):175-82. doi: 10.1042/bj2090175.
本文引用的文献
1
Spectra and reaction kinetics of respiratory pigments of homogenized and intact cells.匀浆细胞和完整细胞呼吸色素的光谱及反应动力学
Nature. 1952 Feb 9;169(4293):215-21. doi: 10.1038/169215a0.
2
THE REACTIVITY OF FERROCYTOCHROME C WITH IRON-BINDING LIGANDS.亚铁细胞色素C与铁结合配体的反应活性。
J Biol Chem. 1964 May;239:1504-8.
3
Properties and primary structure of the cytochrome c from the flight muscles of the moth, Samia cynthia.
J Biol Chem. 1966 Jan 25;241(2):335-48.
4
The reaction of cytochrome c with imidazole.细胞色素c与咪唑的反应。
Biochemistry. 1969 Jan;8(1):149-53. doi: 10.1021/bi00829a021.
5
Ferricytochrome c. I. General features of the horse and bonito proteins at 2.8 A resolution.高铁细胞色素c。I. 马和鲣鸟蛋白质在2.8埃分辨率下的一般特征。
J Biol Chem. 1971 Mar 10;246(5):1511-35.
6
One-electron reactions in biochemical systems as studied by pulse radiolysis. V. Cytochrome c.脉冲辐解研究生化系统中的单电子反应。V. 细胞色素c。
Arch Biochem Biophys. 1971 Jul;145(1):365-72. doi: 10.1016/0003-9861(71)90049-x.
7
Theoretical and experimental studies of the electron transfer reactions in the cytochrome chain. II. Fitting of the experimental data.
Arch Biochem Biophys. 1971 Dec;147(2):675-82. doi: 10.1016/0003-9861(71)90427-9.
8
On the elucidation of the pH dependence of the oxidation-reduction potential of cytochrome c at alkaline pH.关于细胞色素c在碱性pH值下氧化还原电位的pH依赖性的阐明。
J Biol Chem. 1966 Sep 25;241(18):4180-5.
9
Electron transfer to ferricytochrome c: reaction with hydrated electrons and conformational transitions involved.电子向高铁细胞色素c的转移：与水合电子的反应及相关构象转变。
Proc Natl Acad Sci U S A. 1972 Apr;69(4):902-6. doi: 10.1073/pnas.69.4.902.
10
Mechanisms of the reactions of cytochrome c. II. The rate of reduction of horse-heart ferricytochrome c by chromium(II).细胞色素c的反应机制。II. 铬（II）对马心铁细胞色素c的还原速率。
J Am Chem Soc. 1973 Feb 21;95(4):1131-7. doi: 10.1021/ja00785a022.
Zotero 插件