用光反应性糖脂探针绘制新城疫病毒的膜蛋白图谱。
Mapping the membrane proteins of Newcastle-disease virus with a photoreactive glycolipid probe.
作者信息
Bramhall J S, Shiflett M A, Wisnieski B J
出版信息
Biochem J. 1979 Feb 1;177(2):765-8. doi: 10.1042/bj1770765.
Abstract
The envelope proteins of Newcastle-disease virus were preferentially labelled when a suspension of virus particles that contained the photoreactive probe 12-(4-azido-2-nitro-phenoxy)stearoyl[1-14C]glucosamine was irradiated. One of the proteins labelled was not readily accessible to surface labelling with 125I.
摘要
当含有光反应性探针12-(4-叠氮基-2-硝基苯氧基)硬脂酰基[1-¹⁴C]葡糖胺的病毒颗粒悬液受到照射时，新城疫病毒的包膜蛋白被优先标记。其中一种被标记的蛋白不易被¹²⁵I进行表面标记。
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