溶血磷脂酰胆碱可以调节来自大鼠肝细胞膜的胰高血糖素刺激的腺苷酸环化酶的活性。
Lysophosphatidylcholines can modulate the activity of the glucagon-stimulated adenylate cyclase from rat liver plasma membranes.
作者信息
Houslay M D, Palmer R W
出版信息
Biochem J. 1979 Jan 15;178(1):217-21. doi: 10.1042/bj1780217.
Abstract
- Synthetic lysophosphatidylcholines inhibit the glucagon-stimulated adenylate cyclase activity of rat liver plasma membranes at concentrations two to five times lower than those needed to inhibit the fluoride-stimulated activity. 2. Specific 125I-labelled glucagon binding to hormone receptors is inhibited at concentrations similar to those inhibiting the fluoride-stimulated activity. 3. At concentrations of lysophosphatidylcholines immediately below those causing inhibition, an activation of adenylate cyclase activity or hormone binding was observed. 4 These effects are essentially reversible. 5. We conclude that the increased sensitivity of glucagon-stimulated adenylate cyclase to inhibition may be due to the lysophosphatidylcholines interfering with the physical coupling between the hormone receptor and catalytic unit of adenylate cyclase. 6. We suggest that, in vivo, it is possible that lysophosphatidylcholines may modulate the activity of adenylate cyclase only when it is in the hormone-stimulated state.
摘要
- 合成溶血磷脂酰胆碱抑制大鼠肝细胞膜中胰高血糖素刺激的腺苷酸环化酶活性时所需的浓度，比抑制氟化物刺激的活性所需浓度低两到五倍。2. 特异性125I标记的胰高血糖素与激素受体的结合在与抑制氟化物刺激活性相似的浓度下受到抑制。3. 在溶血磷脂酰胆碱浓度略低于引起抑制的浓度时，观察到腺苷酸环化酶活性或激素结合的激活。4. 这些效应基本上是可逆的。5. 我们得出结论，胰高血糖素刺激的腺苷酸环化酶对抑制的敏感性增加可能是由于溶血磷脂酰胆碱干扰了激素受体与腺苷酸环化酶催化单位之间的物理偶联。6. 我们认为，在体内，溶血磷脂酰胆碱可能仅在处于激素刺激状态时才调节腺苷酸环化酶的活性。
相似文献
1
Lysophosphatidylcholines can modulate the activity of the glucagon-stimulated adenylate cyclase from rat liver plasma membranes.溶血磷脂酰胆碱可以调节来自大鼠肝细胞膜的胰高血糖素刺激的腺苷酸环化酶的活性。
Biochem J. 1979 Jan 15;178(1):217-21. doi: 10.1042/bj1780217.
2
The activity of glucagon-stimulated adenylate cyclase from rat liver plasma membranes is modulated by the fluidity of its lipid environment.来自大鼠肝细胞膜的胰高血糖素刺激的腺苷酸环化酶的活性受其脂质环境流动性的调节。
Biochem J. 1978 Jul 15;174(1):179-90. doi: 10.1042/bj1740179.
3
In vitro comparisons of hepatic adenylate cyclase from normal and hypophysectomized rat liver plasma membrane.
Endocrinology. 1984 Apr;114(4):1155-62. doi: 10.1210/endo-114-4-1155.
4
Elevated membrane cholesterol concentrations inhibit glucagon-stimulated adenylate cyclase.膜胆固醇浓度升高会抑制胰高血糖素刺激的腺苷酸环化酶。
Biochem J. 1983 Feb 15;210(2):437-49. doi: 10.1042/bj2100437.
5
Acidic phospholipid species inhibit adenylate cyclase activity in rat liver plasma membranes.酸性磷脂种类抑制大鼠肝细胞膜中的腺苷酸环化酶活性。
Biochem J. 1986 Apr 1;235(1):237-43. doi: 10.1042/bj2350237.
6
Modulation of adenylate cyclase activity in liver and fat cell membranes by insulin.胰岛素对肝细胞膜和脂肪细胞膜中腺苷酸环化酶活性的调节作用。
Science. 1972 Feb 25;175(4024):906-8. doi: 10.1126/science.175.4024.906.
7
Guanosine 5'-triphosphate and guanosine 5'-[beta gamma-imido]triphosphate effect a collision coupling mechanism between the glucagon receptor and catalytic unit of adenylate cyclase.5'-三磷酸鸟苷和5'-[βγ-亚氨基]三磷酸鸟苷在胰高血糖素受体与腺苷酸环化酶催化单元之间实现了一种碰撞偶联机制。
Biochem J. 1980 Mar 15;186(3):649-58. doi: 10.1042/bj1860649.
8
Glucagon-stimulated adenylate cyclase detects a selective perturbation of the inner half of the liver plasma-membrane bilayer achieved by the local anaesthetic prilocaine.胰高血糖素刺激的腺苷酸环化酶检测到局部麻醉药丙胺卡因对肝细胞膜双层内侧一半造成的选择性扰动。
Biochem J. 1980 Jul 15;190(1):131-7. doi: 10.1042/bj1900131.
9
Metformin treatment of lean and obese Zucker rats modulates the ability of glucagon and insulin to regulate hepatocyte adenylate cyclase activity.二甲双胍对瘦型和肥胖型 Zucker 大鼠的治疗可调节胰高血糖素和胰岛素调节肝细胞腺苷酸环化酶活性的能力。
J Endocrinol. 1989 Jul;122(1):207-12. doi: 10.1677/joe.0.1220207.
10
The effect of fluoride on the state of aggregation of adenylate cyclase in rat liver plasma membranes.氟化物对大鼠肝细胞膜中腺苷酸环化酶聚集状态的影响。
Biochem J. 1980 Apr 15;188(1):137-40. doi: 10.1042/bj1880137.
引用本文的文献
1
Effect of phosphatidylcholine structure on the adenylate cyclase activity of a murine fibroblast cell line.磷脂酰胆碱结构对小鼠成纤维细胞系腺苷酸环化酶活性的影响。
Lipids. 1993 Aug;28(8):727-30. doi: 10.1007/BF02535994.
2
Phospholipid modifications and adenylate cyclase in rat liver plasma membranes.大鼠肝细胞膜中的磷脂修饰与腺苷酸环化酶
Agents Actions. 1981 Dec;11(6-7):547-9. doi: 10.1007/BF01978733.
3
Phenobarbital selectively modulates the glucagon-stimulated activity of adenylate cyclase by depressing the lipid phase separation occurring in the outer half of the bilayer of liver plasma membranes.苯巴比妥通过抑制肝细胞膜双层外半部分发生的脂质相分离，选择性地调节胰高血糖素刺激的腺苷酸环化酶活性。
Biochem J. 1981 Sep 1;197(3):675-81. doi: 10.1042/bj1970675.
4
Endotoxin inhibits the fluoride-stimulated adenylate cyclase activity of rat liver membranes enriched with bile canaliculi.内毒素抑制富含胆小管的大鼠肝细胞膜中氟化物刺激的腺苷酸环化酶活性。
Experientia. 1982 Jul 15;38(7):831-3. doi: 10.1007/BF01972299.
5
Glucagon-stimulated adenylate cyclase detects a selective perturbation of the inner half of the liver plasma-membrane bilayer achieved by the local anaesthetic prilocaine.胰高血糖素刺激的腺苷酸环化酶检测到局部麻醉药丙胺卡因对肝细胞膜双层内侧一半造成的选择性扰动。
Biochem J. 1980 Jul 15;190(1):131-7. doi: 10.1042/bj1900131.
6
Guanosine 5'-triphosphate and guanosine 5'-[beta gamma-imido]triphosphate effect a collision coupling mechanism between the glucagon receptor and catalytic unit of adenylate cyclase.5'-三磷酸鸟苷和5'-[βγ-亚氨基]三磷酸鸟苷在胰高血糖素受体与腺苷酸环化酶催化单元之间实现了一种碰撞偶联机制。
Biochem J. 1980 Mar 15;186(3):649-58. doi: 10.1042/bj1860649.
7
Acidic phospholipid species inhibit adenylate cyclase activity in rat liver plasma membranes.酸性磷脂种类抑制大鼠肝细胞膜中的腺苷酸环化酶活性。
Biochem J. 1986 Apr 1;235(1):237-43. doi: 10.1042/bj2350237.
8
Sialylation of lacto-N-neotetraosyl ceramide by a solubilized sialyltransferase(s) from chicken skeletal muscle: effect of phosphatidylcholine and sphingomyelin.来自鸡骨骼肌的可溶性唾液酸转移酶对乳糖-N-新四糖神经酰胺的唾液酸化作用：磷脂酰胆碱和鞘磷脂的影响。
Lipids. 1989 Jun;24(6):550-4. doi: 10.1007/BF02535137.
本文引用的文献
1
A micro biuret method for protein determination; determination of total protein in cerebrospinal fluid.一种用于蛋白质测定的微量双缩脲法；脑脊液中总蛋白的测定
Scand J Clin Lab Invest. 1953;5(3):218-22. doi: 10.3109/00365515309094189.
2
Effects of ethylenic bond position upon acyltransferase activity with isomeric cis-octadecenoyl coenzyme A thiol esters.双键位置对异构顺式十八碳烯酰辅酶A硫醇酯的酰基转移酶活性的影响。
Biochim Biophys Acta. 1969 Apr 29;176(3):480-90. doi: 10.1016/0005-2760(69)90215-x.
3
The assembly of ribosomes in yeast.酵母中核糖体的组装。
J Biol Chem. 1971 Jan 25;246(2):447-54.
4
Synthesis of lecithins by acylation of O-(sn-glycero-3-phosphoryl) choline with fatty acid anhydrides.通过用脂肪酸酐酰化O-(sn-甘油-3-磷酸)胆碱合成卵磷脂。
Biochim Biophys Acta. 1969 Dec 17;187(4):520-6. doi: 10.1016/0005-2760(69)90049-6.
5
Selective transfers of trans-ethylenic acids by acyl coenzyme A. Phospholipid acyltransferases.通过酰基辅酶A进行反式烯酸的选择性转移。磷脂酰基转移酶。
Biochemistry. 1972 Nov 7;11(23):4392-8. doi: 10.1021/bi00773a028.
6
Stimulation of galactosyltransferase in liver microsomes by lysolecithin.溶血卵磷脂对肝微粒体中半乳糖基转移酶的刺激作用。
Biochem Biophys Res Commun. 1974 Apr 8;57(3):815-22. doi: 10.1016/0006-291x(74)90619-6.
7
Synthetic I-acylglycerophospholipid analogues as lipid substrates. I. Studies on microsomal acylation and uptake into liver slices of I-acyl analogues containing various polar bases.
Biochim Biophys Acta. 1973 Jan 19;296(1):24-33. doi: 10.1016/0005-2760(73)90040-4.
8
Acyl coenzyme A:1-acylglycerophosphorylglycerol acyltransferase from rat liver.大鼠肝脏中的酰基辅酶A：1-酰基甘油磷酸甘油酰基转移酶
J Biol Chem. 1973 Apr 25;248(8):2906-11.
9
Phosphoglyceride metabolism.磷酸甘油酯代谢
Annu Rev Biochem. 1974;43(0):243-77. doi: 10.1146/annurev.bi.43.070174.001331.
10
Effects of glucagon on cyclic AMP and carbohydrate metabolism in livers from diabetic rats.胰高血糖素对糖尿病大鼠肝脏中环磷酸腺苷及碳水化合物代谢的影响。
Biochim Biophys Acta. 1974 Mar 20;343(1):250-67. doi: 10.1016/0304-4165(74)90258-x.
Zotero 插件