Savart M
Biochim Biophys Acta. 1979 Mar 28;562(1):131-8. doi: 10.1016/0005-2787(79)90132-1.
A transortin-hydrocortisone complex has been isolated from human serum by affinity chromatography on oxidized corticosterone coupled to AH-Sepharose 4B. The influence of this complex and of hydrocortisone alone on endogenous RNA polymerase activity from thymus chromatin have been tested. Results show that hydrocortisone alone has no effect on RNA polymerase activity from thymus chromatin. Under the same experimental conditions, The transcortin-hydrocortisone complex induces an important decrease in the incorporation of UMP into RNA. The dose response of thymic RNA polymerase to transcortin-hydrocortisone complex and the effects of alpha-amanitin on this response are also reported.
通过在与AH-琼脂糖4B偶联的氧化皮质酮上进行亲和层析，从人血清中分离出一种运皮质素-氢化可的松复合物。已经测试了这种复合物以及单独的氢化可的松对胸腺染色质内源RNA聚合酶活性的影响。结果表明，单独的氢化可的松对胸腺染色质的RNA聚合酶活性没有影响。在相同的实验条件下，运皮质素-氢化可的松复合物导致UMP掺入RNA的量显著减少。还报道了胸腺RNA聚合酶对运皮质素-氢化可的松复合物的剂量反应以及α-鹅膏蕈碱对该反应的影响。
