Brèthes D, Chevallier J, Tenu J P
Biochimie. 1979;61(1):109-13. doi: 10.1016/s0300-9084(79)80319-3.
We have studied, under different conditions and through the hydrolysis of p-nitrophenylphosphate, the stoechiometry of the active transport of calcium by sarcoplasmic reticulum. According to a minimal kinetic model, it is possible to explain the variation of the coupling factor n(t) during the calcium accumulation in absence of precipitating agent. Under the described experimental conditions, at the beginning of the experiment, the coupling factor's value is slightly above 2.
我们通过对磷酸对硝基苯酯的水解反应,在不同条件下研究了肌质网对钙的主动转运的化学计量关系。根据一个最小动力学模型,在不存在沉淀剂的情况下钙积累过程中偶联因子n(t)的变化是可以解释的。在所描述的实验条件下,在实验开始时,偶联因子的值略高于2。