Barnes R, Colleran E M, Jones O T
Biochem J. 1973 Jul;134(3):745-51. doi: 10.1042/bj1340745.
A method is described for the preparation of mitochondria from the slime mould Physarum polycephalum; the mitochondria were not coupled. P. polycephalum mitochondria oxidized added NADH via a rotenone-insensitive pathway, but the oxidation of malate plus glutamate was rotenone sensitive; both of these substrates reduced much less cytochrome b than did succinate, in both aerobic and anaerobic steady states. Spectroscopy at 77 degrees K separated three absorption maxima in the alpha-band region, at 560nm, 553nm and one at 547nm due to cytochrome c. The absorption at 553nm was increased in the aerobic steady state by the addition of 2-heptyl-4-hydroxyquinoline N-oxide, suggesting that it was due to a b-type cytochrome. All three absorption maxima appeared in the aerobic steady state after the addition of a range of substrates. The respiratory activity with different substrates and the response to inhibitors of respiration were similar to those previously described for fungus mitochondria (Weiss et al., 1970; Erickson & Ashworth, 1969). When grown under conditions of haem limitation the mitochondria contained a lower concentration of cytochromes than normal.
本文描述了一种从多头绒泡菌制备线粒体的方法；所制备的线粒体未发生偶联。多头绒泡菌线粒体通过对鱼藤酮不敏感的途径氧化添加的NADH，但苹果酸加谷氨酸的氧化对鱼藤酮敏感；在需氧和厌氧稳定状态下，这两种底物使细胞色素b的还原程度均远低于琥珀酸。77K光谱在α波段区域分离出三个吸收最大值，分别位于560nm、553nm以及由于细胞色素c产生的一个位于547nm处的吸收峰。在需氧稳定状态下，添加2-庚基-4-羟基喹啉N-氧化物会使553nm处的吸收增加，这表明它归因于一种b型细胞色素。添加一系列底物后，在需氧稳定状态下出现了所有三个吸收最大值。不同底物的呼吸活性以及对呼吸抑制剂的反应与先前针对真菌线粒体所描述的相似（Weiss等人，1970年；Erickson和Ashworth，1969年）。当在血红素限制条件下生长时，线粒体中细胞色素的浓度低于正常水平。
