Tierney J T, Sullivan R, Larkin E P, Peeler J T
Appl Microbiol. 1973 Oct;26(4):497-501. doi: 10.1128/am.26.4.497-501.1973.
Various methods for the recovery of virus inoculated into ground beef were investigated in an attempt to develop a sensitive system that could be used to detect viral contaminants in market foods. A 100-g sample, inoculated with poliovirus 1, was suspended in 150 to 900 ml of Eagle minimum essential medium, pH 8.5, and mixed in either plastic bags or plastic cups on a mechanical shaker. The particulate materials were removed by means of cheese cloth, glass wool, woven fiber glass, or low-speed centrifugation. Large volumes of fluid were concentrated by ultrafiltration. Microbiological contamination was controlled by high antibiotic concentrations or by filtration. Quantitative plaque-forming-unit recovery of the virus was determined by utilizing an agar overlay technique on Vero cell cultures. The data indicated that from 20 to 50% of the seeded virus could be recovered from a 100-g sample of ground beef. The glass wool and woven fiber glass methods were the most effective, with recovery of approximately 50% of the inoculated virus.
为了开发一种可用于检测市售食品中病毒污染物的灵敏系统，对多种从接种于绞碎牛肉中的病毒进行回收的方法展开了研究。将接种了脊髓灰质炎病毒1型的100克样品悬浮于150至900毫升pH值为8.5的伊格尔最低限度基本培养基中，并在塑料袋或塑料杯中通过机械振荡器进行混合。通过粗棉布、玻璃棉、玻璃纤维织物或低速离心去除颗粒物质。通过超滤浓缩大量液体。通过高浓度抗生素或过滤控制微生物污染。利用Vero细胞培养物上的琼脂覆盖技术测定病毒的定量空斑形成单位回收率。数据表明，从100克绞碎牛肉样品中可回收20%至50%的接种病毒。玻璃棉和玻璃纤维织物方法最为有效，可回收约50%的接种病毒。
