Klessen C
Blut. 1979 Apr 20;38(4):343-8. doi: 10.1007/BF01008149.
Using a modified colloidal iron reaction two positively reacting components in neutrophil leukocytes are discernible: 1. In neutrophils of unfixed smears the outer membrane or surface coat is stained. 2. After fixation with buffered formalin, formalin-sublimate or Helly's fluid a strongly reacting cytoplasmic component is demonstrable. After fixation with formalin-sublimate or Helly's fluid the latter has been proven to be sensitive against treatment with sialidase thus indicating the presence of sialic acid residues in neutrophil granulocytes.
使用改良的胶体铁反应,可在中性粒细胞中辨别出两种呈阳性反应的成分:1. 在未固定涂片的中性粒细胞中,外膜或表面被染色。2. 用缓冲福尔马林、福尔马林 - 升汞或赫利氏液固定后,可显示出一种强烈反应的细胞质成分。在用福尔马林 - 升汞或赫利氏液固定后,已证明后者对唾液酸酶处理敏感,因此表明中性粒细胞中存在唾液酸残基。