甲状腺激素对大鼠心脏和骨骼肌微粒体制剂中特异性[3H]-哇巴因结合的刺激作用：6-羟基多巴胺的影响

Stimulation of specific [3H]-ouabain binding to microsomal preparations from rat heart and skeletal muscle by thyroid hormones: effects of 6-hydroxydopamine.

Banerjee S P, Sharma V K

Br J Pharmacol. 1979 Apr;65(4):615-21. doi: 10.1111/j.1476-5381.1979.tb07872.x.

Abstract

Surgical thyroidectomy decreased specific [3H]-ouabain binding to heart ventricular microsomes by 43% and gastrocnemius muscle microsomes by 34%. Administration of triiodothyronine to euthyroid rats enhanced specific [3H]-ouabain binding to heart and skeletal muscle membrane by 60% and 33% respectively. 2. Treatment of thyroidectomized rats with triiodothyronine increased specific [3H]-ouabain binding by 44% in skeletal muscle membrane preparation and 428% in cardiac microsomes. 3. Specific [3H]-ouabain binding decreased by 55% in heart and 53% in gastrocnemius muscle preparations following chemical sympathectomy with 6-hydroxydopamine. 4. Treatment with triiodothyronine of euthyroid rats which had been sympathectomized did not significantly alter specific [3H]-ouabain binding to heart or skeletal muscle membrane preparations. 5. Administration of triiodothyronine to thyroidectomized and sympathectomized rats increased specific [3H]-ouabain binding by 80% in heart and 83% in skeletal muscle membrane preparations. 6. These results suggest that triiodothyronine may influence specific [3H]-ouabain binding to thyroid hormone nonresponsive tissue such as sympathetic nerve endings. Therefore, the present observations are incompatible with the hypothesis that induction of (Na+ +K+)-adenosine triphosphatase of skeletal muscle membrane is the molecular mechanism for the calorigenic actions of thyroid hormones.

摘要

手术切除甲状腺后，心脏心室微粒体上特异性[3H]哇巴因结合减少了43%，腓肠肌微粒体上减少了34%。给甲状腺功能正常的大鼠注射三碘甲状腺原氨酸后，心脏和骨骼肌膜上的特异性[3H]哇巴因结合分别增加了60%和33%。2. 用三碘甲状腺原氨酸治疗甲状腺切除的大鼠，骨骼肌膜制剂中特异性[3H]哇巴因结合增加了44%，心脏微粒体中增加了428%。3. 用6-羟基多巴胺进行化学性交感神经切除后，心脏制剂中特异性[3H]哇巴因结合减少了55%，腓肠肌制剂中减少了53%。4. 用三碘甲状腺原氨酸治疗已进行交感神经切除的甲状腺功能正常的大鼠，对心脏或骨骼肌膜制剂上的特异性[3H]哇巴因结合没有显著影响。5. 给甲状腺切除且交感神经切除的大鼠注射三碘甲状腺原氨酸，心脏膜制剂中特异性[3H]哇巴因结合增加了80%，骨骼肌膜制剂中增加了83%。6. 这些结果表明，三碘甲状腺原氨酸可能影响特异性[3H]哇巴因与甲状腺激素无反应组织（如交感神经末梢）的结合。因此，目前的观察结果与骨骼肌膜(Na++K+)-ATP酶的诱导是甲状腺激素产热作用的分子机制这一假说不一致。