细胞质雌激素受体的薄层凝胶过滤分析。一种用于激素依赖性肿瘤的可能筛选方法。
A thin-layer gel filtration assay of cytoplasmic oestrogen receptors. A possible screening method for hormone dependent tumours.
作者信息
Gore-Langton R E, Ashwood-Smith M J, Algard F T, van Netten J P
出版信息
Br J Cancer. 1973 Oct;28(4):310-5. doi: 10.1038/bjc.1973.153.
Abstract
A thin-layer gel filtration system for the assay of oestrogen receptors in target organ tissue samples as small as 50 mg is presented and compared with the sucrose gradient centrifugation method. Only specific high-affinity binding of [(3)H]17β-oestradiol to endometrial cytosol has been observed with the thin-layer gel filtration assay, despite the presence of relatively high levels of nonspecific binding components. The system could be adapted to the clinical determination of oestrogen receptor content in biopsy material from mammary and endometrial tumours and would be of value in predicting hormone dependency.
摘要
本文介绍了一种薄层凝胶过滤系统，用于检测小至50毫克的靶器官组织样本中的雌激素受体，并与蔗糖梯度离心法进行了比较。尽管存在相对较高水平的非特异性结合成分，但通过薄层凝胶过滤分析仅观察到[(3)H]17β-雌二醇与子宫内膜细胞质的特异性高亲和力结合。该系统可用于临床测定乳腺和子宫内膜肿瘤活检材料中的雌激素受体含量，对预测激素依赖性具有重要价值。
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A thin-layer gel filtration assay of cytoplasmic oestrogen receptors. A possible screening method for hormone dependent tumours.细胞质雌激素受体的薄层凝胶过滤分析。一种用于激素依赖性肿瘤的可能筛选方法。
Br J Cancer. 1973 Oct;28(4):310-5. doi: 10.1038/bjc.1973.153.
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A receptor molecule for estrogens: isolation from the rat uterus and preliminary characterization.一种雌激素受体分子：从大鼠子宫中分离及初步特性鉴定。
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Estrogen receptors and breast cancer response to adrenalectomy.雌激素受体与乳腺癌对肾上腺切除术的反应。
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