Seemayer N H, Defendi V
J Virol. 1973 Dec;12(6):1265-71. doi: 10.1128/JVI.12.6.1265-1271.1973.
After light UV irradiation (5,000 to 10,000 ergs/mm(2)) "complete" and "defective" simian virus 40 (SV40) showed an enhancement of oncogenic transformation capacity in Syrian hamster kidney cells in vitro up to 180 and 270% of the controls, respectively. Simultaneously with the enhancement of transformation, an increase in T-antigen induction was observed in CV-1 cells infected with light UV-irradiated SV40; infectivity, however, was correspondingly reduced by 1 log(10). After strong UV irradiation (10,000 to 80,000 ergs/mm(2)) of "complete" and "defective" SV40, transformation capacity in vitro proved to be the most resistant viral function. It was only slightly reduced in comparison with a 4 to 5 log(10) reduction of infectivity. T-antigen induction of SV40 was also equally resistant to strong UV irradiation. We found no evidence of "multiplicity reactivation" involved in the high resistance of transformation capacity of SV40 after UV irradiation. Syrian hamster kidney cells transformed in vitro by UV-irradiated SV40 contained the SV40-specific T-antigen and showed the same morphology and growth characteristics as cells transformed by non-irradiated "complete" or "defective" SV40. They induced malignant tumors after subcutaneous inoculation into Syrian hamsters.
轻度紫外线照射（5000至10000尔格/平方毫米）后，“完整”和“缺陷”型猿猴病毒40（SV40）在体外叙利亚仓鼠肾细胞中的致癌转化能力分别增强至对照的180%和270%。在转化能力增强的同时，在用轻度紫外线照射的SV40感染的CV-1细胞中观察到T抗原诱导增加；然而，感染性相应降低了1个对数（10）。对“完整”和“缺陷”型SV40进行强烈紫外线照射（10000至80000尔格/平方毫米）后，体外转化能力被证明是最具抗性的病毒功能。与感染性降低4至5个对数（10）相比，它仅略有降低。SV40的T抗原诱导对强烈紫外线照射也同样具有抗性。我们没有发现紫外线照射后SV40转化能力的高抗性涉及“多重复活”的证据。经紫外线照射的SV40在体外转化的叙利亚仓鼠肾细胞含有SV40特异性T抗原，并且表现出与未照射的“完整”或“缺陷”型SV40转化的细胞相同的形态和生长特征。将它们皮下接种到叙利亚仓鼠中后可诱发恶性肿瘤。
