Sinensky M
Proc Natl Acad Sci U S A. 1974 Feb;71(2):522-5. doi: 10.1073/pnas.71.2.522.
E. coli incorporates increasing proportions of saturated and long-chain fatty acids into phospholipids as growth temperature is increased. It was found that this compositional variation results in the biosynthesis of phospholipids that have identical viscosities at the temperature of growth of the cells. This "homeoviscous adaptation" can also be observed in E. coli membrane preparations. Viscosities were determined by use of the electron spin resonance spin-label technique.
随着生长温度升高,大肠杆菌将越来越多比例的饱和脂肪酸和长链脂肪酸纳入磷脂中。研究发现,这种组成变化导致在细胞生长温度下具有相同粘度的磷脂生物合成。这种“等粘度适应”在大肠杆菌膜制剂中也能观察到。粘度通过电子自旋共振自旋标记技术测定。