Fakan F
Cesk Patol. 1979 Feb;15(1):1-6.
Selected cytoenzymological methods together with the PAS reaction often permit more precise identifying normal and tumorous blood cells and thus determining the type of hemoblastosis. The cells of the myeloid neutrophil series are characterized by the activity of peroxidase and naphtol-AS-D-chloracetate esterase, monocytic elements by the activity of a nonspecific esterase sensitive to fluoride, the tumorous cells of the red series by the PAS positivity and paranuclear positivity of acid phosphatase. Some tumorous lymphoblasts have gross PAS--positive granules in the plasma, others, probably of T-origin, paranuclear focal positivity of acid phosphatase. Hematological classification of acute hemoblastoses does not exactly correspond to Löffler's cytochemical classification; of diagnostic and therapeutically considerable importance is the synthetic evaluation of the hematological finding and of interpretive possibilities of individual cytochemical methods.
选定的细胞酶学方法与PAS反应一起，常常能更精确地识别正常血细胞和肿瘤血细胞，从而确定成血病的类型。髓系中性粒细胞系列的细胞以过氧化物酶和萘酚-AS-D-氯乙酸酯酶的活性为特征，单核细胞成分以对氟敏感的非特异性酯酶的活性为特征，红系肿瘤细胞以PAS阳性和酸性磷酸酶的核旁阳性为特征。一些肿瘤性淋巴母细胞在胞浆中有粗大的PAS阳性颗粒，另一些可能起源于T细胞的肿瘤性淋巴母细胞则有酸性磷酸酶的核旁局灶性阳性。急性成血病的血液学分类与勒夫勒的细胞化学分类并不完全一致；血液学检查结果与各个细胞化学方法的解释可能性的综合评估具有重要的诊断和治疗意义。
