用于部分纯化多瘤病毒感染的小鼠细胞中新合成蛋白质的免疫化学方法。

Paulin D, Perreau J, Cuzin F

J Virol. 1974 Mar;13(3):699-705. doi: 10.1128/JVI.13.3.699-705.1974.

A broad-spectrum antiserum has been obtained against nuclear proteins from normal mouse fibroblasts. Solid phase immunoadsorbants prepared from the serum specifically bind the corresponding antigens. The removal in this way of a significant fraction of host proteins leads to partial purification of viral and virus-induced polypeptides from polyoma virus-infected cell extracts. Four main peptides can be selected, with respective molecular weights of 90,000, 70,000, 46,000, and 41,000.

已获得一种针对正常小鼠成纤维细胞核蛋白的广谱抗血清。用该血清制备的固相免疫吸附剂能特异性结合相应抗原。通过这种方式去除大部分宿主蛋白，可从多瘤病毒感染的细胞提取物中部分纯化病毒和病毒诱导的多肽。可以选出四种主要肽段，其分子量分别为90,000、70,000、46,000和41,000。